SIRT1 Activity in Peripheral Blood Mononuclear Cells Correlates with Altered Lung Function in Patients with Chronic Obstructive Pulmonary Disease
Joint Authors
Filippelli, Amelia
Selleri, Carmine
Conti, V.
Manzo, V.
Cotugno, Roberta
Marino, Luigi
Malangone, Paola
Vitale, Carolina
Maglio, Angelantonio
Capaccio, Damiano
Vatrella, Alessandro
Stellato, Cristiana
Corbi, Graziamaria
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-09
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background.
Oxidative stress is a recognized pathogenic mechanism in chronic obstructive pulmonary disease (COPD).
Expression of the NAD+-dependent deacetylase Sirtuin 1 (SIRT1), an antiaging molecule with a key role in oxidative stress response, has been described as decreased in the lung of COPD patients.
No studies so far investigated whether systemic SIRT1 activity was associated to decreased lung function in this disease.
Methods.
We measured SIRT1 protein expression and activity in peripheral blood mononuclear cells (PBMCs) and total oxidative status (TOS), total antioxidant capacity (TEAC), and oxidative stress index (TOS/TEAC) in the plasma of 25 COPD patients, 20 healthy nonsmokers (HnS), and 20 healthy smokers (HS).
Results.
The activity of SIRT1 was significantly lower in COPD patients compared to both control groups while protein expression decreased progressively (HnS > HS > COPD).
TOS levels were significantly lower in HnS than in smoke-associated subjects (COPD and HS), while TEAC levels were progressively lower according (HnS > HS > COPD).
In COPD patients, SIRT1 activity, but not protein levels, correlated significantly with both lung function parameters (FEV1/FVC and FEV1) and TEAC.
Conclusions.
These findings suggest loss of SIRT1-driven antioxidant activity as relevant in COPD pathogenesis and identify SIRT1 activity as a potential convenient biomarker for identification of mild/moderate, stable COPD.
American Psychological Association (APA)
Conti, V.& Corbi, Graziamaria& Manzo, V.& Malangone, Paola& Vitale, Carolina& Maglio, Angelantonio…[et al.]. 2018. SIRT1 Activity in Peripheral Blood Mononuclear Cells Correlates with Altered Lung Function in Patients with Chronic Obstructive Pulmonary Disease. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1212377
Modern Language Association (MLA)
Conti, V.…[et al.]. SIRT1 Activity in Peripheral Blood Mononuclear Cells Correlates with Altered Lung Function in Patients with Chronic Obstructive Pulmonary Disease. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1212377
American Medical Association (AMA)
Conti, V.& Corbi, Graziamaria& Manzo, V.& Malangone, Paola& Vitale, Carolina& Maglio, Angelantonio…[et al.]. SIRT1 Activity in Peripheral Blood Mononuclear Cells Correlates with Altered Lung Function in Patients with Chronic Obstructive Pulmonary Disease. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1212377
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1212377