5-Aminolevulinic Acid-Based Photodynamic Therapy Pretreatment Mitigates Ultraviolet A-Induced Oxidative Photodamage
Joint Authors
Liu, Juan
Luo, Dan
Hua, Hui
Cheng, Jiawei
Bu, Wenbo
Ma, Weiwei
Si, Chenchen
Wang, Jie
Zhou, Bing-rong
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-07
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Aim.
To determine whether 5-aminolevulinic acid-based photodynamic therapy (ALA-PDT) is effective in combating ultraviolet A- (UVA-) induced oxidative photodamage of hairless mice skin in vivo and human epidermal keratinocytes in vitro.
Methods.
In in vitro experiments, the human keratinocyte cell line (HaCaT cells) was divided into two groups: the experimental group was treated with ALA-PDT and the control group was left untreated.
Then, the experimental group and the control group of cells were exposed to 10 J/m2 of UVA radiation.
ROS, O2− species, and MMP were determined by fluorescence microscopy; p53, OGG1, and XPC were determined by Western blot analysis; apoptosis was determined by flow cytometry; and 8-oxo-dG was determined by immunofluorescence.
Moreover, HaCaT cells were also treated with ALA-PDT.
Then, SOD1 and SOD2 were examined by Western blot analysis.
In in vivo experiments, the dorsal skin of hairless mice was treated with ALA-PDT or saline-PDT, and then, they were exposed to 20 J/m2 UVA light.
The compound 8-oxo-dG was detected by immunofluorescence.
Conclusion.
In human epidermal keratinocytes and hairless mice skin, UVA-induced oxidative damage can be prevented effectively with ALA-PDT pretreatment.
American Psychological Association (APA)
Hua, Hui& Cheng, Jiawei& Bu, Wenbo& Liu, Juan& Ma, Weiwei& Si, Chenchen…[et al.]. 2018. 5-Aminolevulinic Acid-Based Photodynamic Therapy Pretreatment Mitigates Ultraviolet A-Induced Oxidative Photodamage. Oxidative Medicine and Cellular Longevity،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1212381
Modern Language Association (MLA)
Hua, Hui…[et al.]. 5-Aminolevulinic Acid-Based Photodynamic Therapy Pretreatment Mitigates Ultraviolet A-Induced Oxidative Photodamage. Oxidative Medicine and Cellular Longevity No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1212381
American Medical Association (AMA)
Hua, Hui& Cheng, Jiawei& Bu, Wenbo& Liu, Juan& Ma, Weiwei& Si, Chenchen…[et al.]. 5-Aminolevulinic Acid-Based Photodynamic Therapy Pretreatment Mitigates Ultraviolet A-Induced Oxidative Photodamage. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1212381
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1212381