Water-Soluble Polymer Assists N-Methyl-D-Aspartic Acid Receptor 2B siRNA Delivery to Relieve Chronic Inflammatory Pain In Vitro and In Vivo

Joint Authors

Peng, Jie
Ma, Jiahui
Yang, Xue
He, Huan
Wu, Haopeng
Ma, Tongtong
Lu, Jianhua

Source

Pain Research and Management

Issue

Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2018-01-14

Country of Publication

Egypt

No. of Pages

9

Main Subjects

Diseases

Abstract EN

We constructed a water-soluble lipopolymer (WSLP) as a nonviral gene carrier to deliver siRNA targeting NR2B.

The cytotoxicity and serum stability of WSLP loaded with siRNA were evaluated, and the knockdown efficiency of WSLP/NR2B-siRNA in PC12 cells was examined.

The results showed that WSLP could protect the loading siRNAs from enzymatic degradation in serum and exhibit low cytotoxicity to cells.

After transfection, WSLP/NR2B-siRNA complexes reduced the NR2B transcriptional level by 50% and protein level by 55% compared to control siRNA.

Moreover, 3 days after intrathecal injection of WSLP/NR2B-siRNA complexes into rats, the NR2B protein expression decreased significantly to 58%, compared to control treatment (p<0.01).

Injection of WSLP with scrambled siRNA or of polyethylenimine (PEI) with NR2B-siRNA did not show this inhibitory effect.

Additionally, injection of WSLP/NR2B-siRNA complexes significantly relieved inflammatory pain in rats at 3, 4, and 5 days with reduced MWT and decreased TWL scores, while injection of WSLP with scrambled siRNA or of PEI with NR2B-siRNA did not.

These results demonstrated that WSLP can efficiently deliver siRNA targeting NR2B to PC12 cells and relieve pain in rats with chronic inflammatory pain.

American Psychological Association (APA)

Peng, Jie& Ma, Jiahui& Yang, Xue& He, Huan& Wu, Haopeng& Ma, Tongtong…[et al.]. 2018. Water-Soluble Polymer Assists N-Methyl-D-Aspartic Acid Receptor 2B siRNA Delivery to Relieve Chronic Inflammatory Pain In Vitro and In Vivo. Pain Research and Management،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1212603

Modern Language Association (MLA)

Peng, Jie…[et al.]. Water-Soluble Polymer Assists N-Methyl-D-Aspartic Acid Receptor 2B siRNA Delivery to Relieve Chronic Inflammatory Pain In Vitro and In Vivo. Pain Research and Management No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1212603

American Medical Association (AMA)

Peng, Jie& Ma, Jiahui& Yang, Xue& He, Huan& Wu, Haopeng& Ma, Tongtong…[et al.]. Water-Soluble Polymer Assists N-Methyl-D-Aspartic Acid Receptor 2B siRNA Delivery to Relieve Chronic Inflammatory Pain In Vitro and In Vivo. Pain Research and Management. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1212603

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1212603