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Gap Junctions Are Involved in the Rescue of CFTR-Dependent Chloride Efflux by Amniotic Mesenchymal Stem Cells in Coculture with Cystic Fibrosis CFBE41o- Cells
Joint Authors
Seia, M.
Di Gioia, Sante
Castellani, Stefano
Zefferino, Roberto
Beccia, Elisa
Angiolillo, Antonella
Casavola, Valeria
Giannone, Valentina
Favia, Maria
Conese, Massimo
Colombo, Carla
Carbone, Annalucia
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-01-11
Country of Publication
Egypt
No. of Pages
14
Abstract EN
We previously found that human amniotic mesenchymal stem cells (hAMSCs) in coculture with CF immortalised airway epithelial cells (CFBE41o- line, CFBE) on Transwell® filters acquired an epithelial phenotype and led to the expression of a mature and functional CFTR protein.
In order to explore the role of gap junction- (GJ-) mediated intercellular communication (GJIC) in this rescue, cocultures (hAMSC : CFBE, 1 : 5 ratio) were studied for the formation of GJIC, before and after silencing connexin 43 (Cx43), a major component of GJs.
Functional GJs in cocultures were inhibited when the expression of the Cx43 protein was downregulated.
Transfection of cocultures with siRNA against Cx43 resulted in the absence of specific CFTR signal on the apical membrane and reduction in the mature form of CFTR (band C), and in parallel, the CFTR-dependent chloride channel activity was significantly decreased.
Cx43 downregulation determined also a decrease in transepithelial resistance and an increase in paracellular permeability as compared with control cocultures, implying that GJIC may regulate CFTR expression and function that in turn modulate airway epithelium tightness.
These results indicate that GJIC is involved in the correction of CFTR chloride channel activity upon the acquisition of an epithelial phenotype by hAMSCs in coculture with CF cells.
American Psychological Association (APA)
Carbone, Annalucia& Zefferino, Roberto& Beccia, Elisa& Casavola, Valeria& Castellani, Stefano& Di Gioia, Sante…[et al.]. 2018. Gap Junctions Are Involved in the Rescue of CFTR-Dependent Chloride Efflux by Amniotic Mesenchymal Stem Cells in Coculture with Cystic Fibrosis CFBE41o- Cells. Stem Cells International،Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1213098
Modern Language Association (MLA)
Carbone, Annalucia…[et al.]. Gap Junctions Are Involved in the Rescue of CFTR-Dependent Chloride Efflux by Amniotic Mesenchymal Stem Cells in Coculture with Cystic Fibrosis CFBE41o- Cells. Stem Cells International No. 2018 (2018), pp.1-14.
https://search.emarefa.net/detail/BIM-1213098
American Medical Association (AMA)
Carbone, Annalucia& Zefferino, Roberto& Beccia, Elisa& Casavola, Valeria& Castellani, Stefano& Di Gioia, Sante…[et al.]. Gap Junctions Are Involved in the Rescue of CFTR-Dependent Chloride Efflux by Amniotic Mesenchymal Stem Cells in Coculture with Cystic Fibrosis CFBE41o- Cells. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1213098
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213098