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Fibrin Glue Enhances Adipose-Derived Stromal Cell Cytokine Secretion and Survival Conferring Accelerated Diabetic Wound Healing
Joint Authors
Hopfner, Ursula
Machens, Hans-Günther
Maan, Zeshaan N.
Aitzetmueller, Matthias M.
Neßbach, Philipp
Hu, Michael S.
Duscher, Dominik
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-12-19
Country of Publication
Egypt
No. of Pages
8
Abstract EN
Introduction.
Although chronic wounds are a major personal and economic burden, treatment options are still limited.
Among those options, adipose-derived stromal cell- (ASC-) based therapies rank as a promising approach but are restricted by the harsh wound environment.
Here we use a commercially available fibrin glue to provide a deliverable niche for ASCs in chronic wounds.
Material and Methods.
To investigate the in vitro effect of fibrin glue, cultivation experiments were performed and key cytokines for regeneration were quantified.
By using an established murine chronic diabetic wound-healing model, we evaluated the influence of fibrin glue spray seeding on cell survival (In Vivo Imaging System, IVIS), wound healing (wound closure kinetics), and neovascularization of healed wounds (CD31 immunohistochemistry).
Results.
Fibrin glue seeding leads to a significantly enhanced secretion of key cytokines (SDF-1, bFGF, and MMP-2) of human ASCs in vitro.
IVIS imaging showed a significantly prolonged murine ASC survival in diabetic wounds and significantly accelerated complete wound closure in the fibrin glue seeded group.
CD31 immunohistochemistry revealed significantly more neovascularization in healed wounds treated with ASCs spray seeded in fibrin glue vs.
ASC injected into the wound bed.
Conclusion.
Although several vehicles have shown to successfully act as cell carrier systems in preclinical trials, regulatory issues have prohibited clinical usage for chronic wounds.
By demonstrating the ability of fibrin glue to act as a carrier vehicle for ASCs, while simultaneously enhancing cellular regenerative function and viability, this study is a proponent of clinical translation for ASC-based therapies.
American Psychological Association (APA)
Hopfner, Ursula& Aitzetmueller, Matthias M.& Neßbach, Philipp& Hu, Michael S.& Machens, Hans-Günther& Maan, Zeshaan N.…[et al.]. 2018. Fibrin Glue Enhances Adipose-Derived Stromal Cell Cytokine Secretion and Survival Conferring Accelerated Diabetic Wound Healing. Stem Cells International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1213145
Modern Language Association (MLA)
Hopfner, Ursula…[et al.]. Fibrin Glue Enhances Adipose-Derived Stromal Cell Cytokine Secretion and Survival Conferring Accelerated Diabetic Wound Healing. Stem Cells International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1213145
American Medical Association (AMA)
Hopfner, Ursula& Aitzetmueller, Matthias M.& Neßbach, Philipp& Hu, Michael S.& Machens, Hans-Günther& Maan, Zeshaan N.…[et al.]. Fibrin Glue Enhances Adipose-Derived Stromal Cell Cytokine Secretion and Survival Conferring Accelerated Diabetic Wound Healing. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1213145
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213145