Effect of Compression Loading on Human Nucleus Pulposus-Derived Mesenchymal Stem Cells
Joint Authors
Shao, Zengwu
Deng, Xiangyu
Liang, Hang
Chen, Sheng
Ma, Kaige
Huang, Donghua
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-10-08
Country of Publication
Egypt
No. of Pages
10
Abstract EN
Purpose.
Mechanical loading plays a vital role in the progression of intervertebral disc (IVD) degeneration, but little is known about the effect of compression loading on human nucleus pulposus-derived mesenchymal stem cells (NP-MSCs).
Thus, this study is aimed at investigating the effect of compression on the biological behavior of NP-MSCs in vitro.
Methods.
Human NP-MSCs were isolated from patients undergoing lumbar discectomy for IVD degeneration and were identified by immunophenotypes and multilineage differentiation.
Then, cells were cultured in the compression apparatus at 1.0 MPa for different times (0 h, 24 h, 36 h, and 48 h).
The viability-, differentiation-, and differentiation-related genes (Runx2, APP, and Col2) and colony formation-, migration-, and stem cell-related proteins (Sox2 and Oct4) were evaluated.
Results.
The results showed that the isolated cells fulfilled the criteria of MSC stated by the International Society for Cellular Therapy (ISCT).
And our results also indicated that compression loading significantly inhibited cell viability, differentiation, colony formation, and migration.
Furthermore, gene expression suggested that compression loading could downregulate the expression of stem cell-related proteins and lead to NP-MSC stemness losses.
Conclusions.
Our results suggested that the biological behavior of NP-MSCs could be inhibited by compression loading and therefore enhanced our understanding on the compression-induced endogenous repair failure of NP-MSCs during IVDD.
American Psychological Association (APA)
Liang, Hang& Chen, Sheng& Huang, Donghua& Deng, Xiangyu& Ma, Kaige& Shao, Zengwu. 2018. Effect of Compression Loading on Human Nucleus Pulposus-Derived Mesenchymal Stem Cells. Stem Cells International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1213155
Modern Language Association (MLA)
Liang, Hang…[et al.]. Effect of Compression Loading on Human Nucleus Pulposus-Derived Mesenchymal Stem Cells. Stem Cells International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1213155
American Medical Association (AMA)
Liang, Hang& Chen, Sheng& Huang, Donghua& Deng, Xiangyu& Ma, Kaige& Shao, Zengwu. Effect of Compression Loading on Human Nucleus Pulposus-Derived Mesenchymal Stem Cells. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1213155
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213155