An Alternative Method for Long-Term Culture of Chicken Embryonic Stem Cell In Vitro
Joint Authors
Lian, Zhengxing
Zhang, Li
Wu, Yenan
Li, Xiang
Wei, Shao
Xing, Yiming
Han, Hongbing
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-09
Country of Publication
Egypt
No. of Pages
14
Abstract EN
Chicken embryonic stem cells (cESCs) obtained from stage X embryos provide a novel model for the study of avian embryonic development.
A new way to maintain cESCs for a long period in vitro still remains unexplored.
We found that the cESCs showed stem cell-like properties in vitro for a long term with the support of DF-1 feeder and basic culture medium supplemented with human basic fibroblast growth factor (hbFGF), mouse stem cell factor (mSCF), and human leukemia inhibitory factor (hLIF).
During the long culture period, the cESCs showed typical ES cell morphology and expressed primitive stem cell markers with a relatively stable proliferation rate and high telomerase activity.
These cells also exhibited the capability to differentiate into cardiac myocytes, smooth muscle cells, neural cells, osteoblast, and adipocyte in vitro.
Chimera chickens were produced by cESCs cultured for 25 passages with this new culture system.
The experiments showed that DF-1 was the optimal feeder and hbFGF was an important factor for maintaining the pluripotency of cESCs in vitro.
American Psychological Association (APA)
Zhang, Li& Wu, Yenan& Li, Xiang& Wei, Shao& Xing, Yiming& Lian, Zhengxing…[et al.]. 2018. An Alternative Method for Long-Term Culture of Chicken Embryonic Stem Cell In Vitro. Stem Cells International،Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1213282
Modern Language Association (MLA)
Zhang, Li…[et al.]. An Alternative Method for Long-Term Culture of Chicken Embryonic Stem Cell In Vitro. Stem Cells International No. 2018 (2018), pp.1-14.
https://search.emarefa.net/detail/BIM-1213282
American Medical Association (AMA)
Zhang, Li& Wu, Yenan& Li, Xiang& Wei, Shao& Xing, Yiming& Lian, Zhengxing…[et al.]. An Alternative Method for Long-Term Culture of Chicken Embryonic Stem Cell In Vitro. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1213282
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213282