The Dynamic DNA Demethylation during Postnatal Neuronal Development and Neural Stem Cell Differentiation
Joint Authors
Shu, Qiang
Tao, Huikang
Li, Xuekun
Xie, Pei
Cao, Yuhang
Shu, Liqi
Li, Liping
Chen, Junchen
Tian, Guangfeng
Zhuang, Yingliang
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-11
Country of Publication
Egypt
No. of Pages
10
Abstract EN
Background.
DNA demethylation, the conversion of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC), plays important roles in diverse biological processes and multiple diseases by regulating gene expression.
Methods.
In this study, utilizing DNA dot blot, immunofluorescence staining, and qRT-PCR, we studied the expression pattern of Tets, the enzymes governing DNA demethylation, and the levels of 5hmC, 5fC, and 5caC during the postnatal neuronal development of mice.
Results.
It was found that 5hmC, 5fC, and 5caC were highly enriched in multiple brain regions and aNSCs and displayed temporal and spatial patterns during postnatal neuronal development and the differentiation of aNSCs.
Consistently, the expression of Tets also exhibited temporal and spatial patterns.
Conclusion.
DNA demethylation displayed dynamic features during postnatal neuronal development and the differentiation of aNSCs of mice, which could contribute to appropriate gene expression.
American Psychological Association (APA)
Tao, Huikang& Xie, Pei& Cao, Yuhang& Shu, Liqi& Li, Liping& Chen, Junchen…[et al.]. 2018. The Dynamic DNA Demethylation during Postnatal Neuronal Development and Neural Stem Cell Differentiation. Stem Cells International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1213285
Modern Language Association (MLA)
Tao, Huikang…[et al.]. The Dynamic DNA Demethylation during Postnatal Neuronal Development and Neural Stem Cell Differentiation. Stem Cells International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1213285
American Medical Association (AMA)
Tao, Huikang& Xie, Pei& Cao, Yuhang& Shu, Liqi& Li, Liping& Chen, Junchen…[et al.]. The Dynamic DNA Demethylation during Postnatal Neuronal Development and Neural Stem Cell Differentiation. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1213285
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213285