Foxc2 and BMP2 Induce OsteogenicOdontogenic Differentiation and Mineralization of Human Stem Cells from Apical Papilla
Joint Authors
Hu, Xiaoli
Li, Junyuan
Zhang, Xiaolei
Zhang, Wen
Zheng, Jianmao
Xu, Meng
Mao, Xueli
Ling, Junqi
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-25
Country of Publication
Egypt
No. of Pages
10
Abstract EN
As a transcription factor regulated by bone morphogenetic protein 2 (BMP2), Forkhead c2 (Foxc2) plays a pivot role in osteogenesis/odontogenesis.
However, the role of Foxc2 and BMP2 in regulating osteo-/odontogenic differentiation and mineralization of stem cells from apical papilla (SCAP) is still uncertain.
In this research, overexpression of Foxc2 gene significantly improved the proliferation of SCAP four days and eight days after transfection, but overexpression of both Foxc2 and BMP2 genes significantly inhibited the proliferation of SCAP eight days after transfection.
RT-qPCR and western blot results indicated that SCAP-Foxc2-BMP2 significantly upregulated osteo-/odontogenic genes and proteins at most of the time points in SCAP after transfection.
Moreover, SCAP-Foxc2-BMP2 formed notably more alkaline phosphatase-positive and alizarin red-positive mineralized nodules than other three group cells sixteen days after transfection.
In conclusion, our findings revealed that Foxc2 and BMP2 synergistically promoted osteo-/odontogenic differentiation and mineralization of SCAP in vitro.
American Psychological Association (APA)
Zhang, Wen& Zhang, Xiaolei& Li, Junyuan& Zheng, Jianmao& Hu, Xiaoli& Xu, Meng…[et al.]. 2018. Foxc2 and BMP2 Induce OsteogenicOdontogenic Differentiation and Mineralization of Human Stem Cells from Apical Papilla. Stem Cells International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1213287
Modern Language Association (MLA)
Zhang, Wen…[et al.]. Foxc2 and BMP2 Induce OsteogenicOdontogenic Differentiation and Mineralization of Human Stem Cells from Apical Papilla. Stem Cells International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1213287
American Medical Association (AMA)
Zhang, Wen& Zhang, Xiaolei& Li, Junyuan& Zheng, Jianmao& Hu, Xiaoli& Xu, Meng…[et al.]. Foxc2 and BMP2 Induce OsteogenicOdontogenic Differentiation and Mineralization of Human Stem Cells from Apical Papilla. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1213287
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213287