Intra-Articular Injection of Alginate-Microencapsulated Adipose Tissue-Derived Mesenchymal Stem Cells for the Treatment of Osteoarthritis in Rabbits

Joint Authors

Kang, Byung-jae
Choi, Seongjae
Kim, Jun-Hyung
Ha, Jeongho
Jeong, Bo-Ing
Jung, Yun Chan
Lee, Geun-Shik
Woo, Heung-Myong

Source

Stem Cells International

Issue

Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2018-06-26

Country of Publication

Egypt

No. of Pages

10

Abstract EN

We investigated the effects of intra-articular injections of alginate-microencapsulated adipose tissue-derived mesenchymal stem cells (ASCs) during osteoarthritis (OA) development in a rabbit model of anterior cruciate ligament transection (ACLT).

We induced OA in mature New Zealand white rabbits by bilateral ACLT.

Stifle joints were categorised into four groups according to intra-articular injection materials.

Alginate microbeads and microencapsulated ASCs were prepared using the vibrational nozzle technology.

Two weeks after ACLT, the rabbits received three consecutive weekly intra-articular injections of 0.9% NaCl, alginate microbeads, ASCs, or microencapsulated ASCs, into each joint.

Nine weeks after ACLT, we euthanised the rabbits and collected bilateral femoral condyles for macroscopic, histological, and immunohistochemical analyses.

Macroscopic evaluation using the modified OA Research Society International (OARSI) score and total cartilage damage score showed that cartilage degradation on the femoral condyle was relatively low in the microencapsulated-ASC group.

Histological analysis of the lateral femoral condyles indicated that microencapsulated ASCs had significant chondroprotective effects.

Immunohistochemically, the expression of MMP-13 after the articular cartilage damage was relatively low in the microencapsulated-ASC-treated stifle joints.

During the development of experimental OA, as compared to ASCs alone, intra-articular injection of microencapsulated ASCs significantly decreased the progression and extent of OA.

American Psychological Association (APA)

Choi, Seongjae& Kim, Jun-Hyung& Ha, Jeongho& Jeong, Bo-Ing& Jung, Yun Chan& Lee, Geun-Shik…[et al.]. 2018. Intra-Articular Injection of Alginate-Microencapsulated Adipose Tissue-Derived Mesenchymal Stem Cells for the Treatment of Osteoarthritis in Rabbits. Stem Cells International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1213306

Modern Language Association (MLA)

Choi, Seongjae…[et al.]. Intra-Articular Injection of Alginate-Microencapsulated Adipose Tissue-Derived Mesenchymal Stem Cells for the Treatment of Osteoarthritis in Rabbits. Stem Cells International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1213306

American Medical Association (AMA)

Choi, Seongjae& Kim, Jun-Hyung& Ha, Jeongho& Jeong, Bo-Ing& Jung, Yun Chan& Lee, Geun-Shik…[et al.]. Intra-Articular Injection of Alginate-Microencapsulated Adipose Tissue-Derived Mesenchymal Stem Cells for the Treatment of Osteoarthritis in Rabbits. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1213306

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1213306