MicroRNA-126 Priming Enhances Functions of Endothelial Progenitor Cells under Physiological and Hypoxic Conditions and Their Therapeutic Efficacy in Cerebral Ischemic Damage
Joint Authors
Yang, Yi
Pan, Qunwen
Liao, Xiaorong
Zheng, Jieyi
Du, Donghui
Ma, Chunlian
Chen, Yanyu
Zhong, Wangtao
Ma, Xiaotang
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-11
Country of Publication
Egypt
No. of Pages
13
Abstract EN
Endothelial progenitor cells (EPCs) have shown the potential for treating ischemic stroke (IS), while microRNA-126 (miR-126) is reported to have beneficial effects on endothelial function and angiogenesis.
In this study, we investigated the effects of miR-126 overexpression on EPCs and explore the efficacy of miR-126-primed EPCs (EPCmiR-126) in treating IS.
The effects of miR-126 overexpression on EPC proliferation, migratory, tube formation capacity, reactive oxygen species (ROS) production, and nitric oxide (NO) generation were determined.
In in vivo study, the effects of EPCmiR-126 on the cerebral blood flow (CBF), neurological deficit score (NDS), infarct volume, cerebral microvascular density (cMVD), and angiogenesis were determined.
Moreover, the levels of circulating EPCs (cEPCs) and their contained miR-126 were measured.
We found (1) miR-126 overexpression promoted the proliferation, migration, and tube formation abilities of EPCs; decreased ROS; and increased NO production of EPCs via activation of PI3K/Akt/eNOS pathway; (2) EPCmiR-126 was more effective than EPCs in attenuating infarct volume and NDS and enhancing cMVD, CBF, and angiogenesis; and (3) infusion of EPCmiR-126 increased the number and the level of miR-126 in cEPCs.
Our data indicate that miR-126 overexpression enhanced the function of EPCs in vitro and in vivo.
American Psychological Association (APA)
Pan, Qunwen& Zheng, Jieyi& Du, Donghui& Liao, Xiaorong& Ma, Chunlian& Yang, Yi…[et al.]. 2018. MicroRNA-126 Priming Enhances Functions of Endothelial Progenitor Cells under Physiological and Hypoxic Conditions and Their Therapeutic Efficacy in Cerebral Ischemic Damage. Stem Cells International،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1213311
Modern Language Association (MLA)
Pan, Qunwen…[et al.]. MicroRNA-126 Priming Enhances Functions of Endothelial Progenitor Cells under Physiological and Hypoxic Conditions and Their Therapeutic Efficacy in Cerebral Ischemic Damage. Stem Cells International No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1213311
American Medical Association (AMA)
Pan, Qunwen& Zheng, Jieyi& Du, Donghui& Liao, Xiaorong& Ma, Chunlian& Yang, Yi…[et al.]. MicroRNA-126 Priming Enhances Functions of Endothelial Progenitor Cells under Physiological and Hypoxic Conditions and Their Therapeutic Efficacy in Cerebral Ischemic Damage. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1213311
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213311