N-Cadherin Upregulation Promotes the Neurogenic Differentiation of Menstrual Blood-Derived Endometrial Stem Cells
Joint Authors
Liu, Yanli
Yang, Fen
Liang, Shengying
Liu, Qing
Fu, Sulei
Wang, Zhenyu
Yang, Ciqing
Lin, Juntang
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-05
Country of Publication
Egypt
No. of Pages
10
Abstract EN
Peripheral nerve injuries are typically caused by either trauma or medical disorders, and recently, stem cell-based therapies have provided a promising treatment approach.
Menstrual blood-derived endometrial stem cells (MenSCs) are considered an ideal therapeutic option for peripheral nerve repair due to a noninvasive collection procedure and their high proliferation rate and immunological tolerance.
Here, we successfully isolated MenSCs and examined their biological characteristics including their morphology, multipotency, and immunophenotype.
Subsequent in vitro studies demonstrated that MenSCs express high levels of neurotrophic factors, such as NT3, NT4, BDNF, and NGF, and are capable of transdifferentiating into glial-like cells under conventional induction conditions.
Moreover, upregulation of N-cadherin (N-cad) mRNA and protein expression was observed after neurogenic differentiation.
In vivo studies clearly showed that N-cad knockdown via in utero electroporation perturbed the migration and maturation of mouse neural precursor cells (NPCs).
Finally, a further transfection assay also confirmed that N-cad upregulation in MenSCs results in the expression of S100.
Collectively, our results confirmed the paracrine effect of MenSCs on neuroprotection as well as their potential for transdifferentiation into glial-like cells and demonstrated that N-cad upregulation promotes the neurogenic differentiation of MenSCs, thereby providing support for transgenic MenSC-based therapy for peripheral nerve injury.
American Psychological Association (APA)
Liu, Yanli& Yang, Fen& Liang, Shengying& Liu, Qing& Fu, Sulei& Wang, Zhenyu…[et al.]. 2018. N-Cadherin Upregulation Promotes the Neurogenic Differentiation of Menstrual Blood-Derived Endometrial Stem Cells. Stem Cells International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1213325
Modern Language Association (MLA)
Liu, Yanli…[et al.]. N-Cadherin Upregulation Promotes the Neurogenic Differentiation of Menstrual Blood-Derived Endometrial Stem Cells. Stem Cells International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1213325
American Medical Association (AMA)
Liu, Yanli& Yang, Fen& Liang, Shengying& Liu, Qing& Fu, Sulei& Wang, Zhenyu…[et al.]. N-Cadherin Upregulation Promotes the Neurogenic Differentiation of Menstrual Blood-Derived Endometrial Stem Cells. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1213325
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213325