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The Efficacy of Graphene Foams for Culturing Mesenchymal Stem Cells and Their Differentiation into Dopaminergic Neurons
Joint Authors
Chattopadhyay, Munmun
Tasnim, Nishat
Thakur, Vikram
Joddar, Binata
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-03
Country of Publication
Egypt
No. of Pages
12
Abstract EN
The implantation of stem cells in vivo is the ideal approach for the restoration of normal life functions, such as replenishing the decreasing levels of affected dopaminergic (DA) neurons during neurodegenerative disease conditions.
However, combining stem cells with biomaterial scaffolds provides a promising strategy for engineering tissues or cellular delivery for directed stem cell differentiation as a means of replacing diseased/damaged tissues.
In this study, mouse mesenchymal stem cells (MSCs) were differentiated into DA neurons using sonic hedgehog, fibroblast growth factor, basic fibroblast growth factor, and brain-derived neurotrophic factor, while they were cultured within collagen-coated 3D graphene foams (GF).
The differentiation into DA neurons within the collagen-coated GF and controls (collagen gels, plastic) was confirmed using β-III tubulin, tyrosine hydroxylase (TH), and NeuN positive immunostaining.
Enhanced expression of β-III tubulin, TH, and NeuN and an increase in the average neurite extension length were observed when cells were differentiated within collagen-coated GF in comparison with collagen gels.
Furthermore, these graphene-based scaffolds were not cytotoxic as MSC seemed to retain viability and proliferated substantially during in vitro culture.
In summary, these results suggest the utility of 3D graphene foams towards the differentiation of DA neurons from MSC, which is an important step for neural tissue engineering applications.
American Psychological Association (APA)
Tasnim, Nishat& Thakur, Vikram& Chattopadhyay, Munmun& Joddar, Binata. 2018. The Efficacy of Graphene Foams for Culturing Mesenchymal Stem Cells and Their Differentiation into Dopaminergic Neurons. Stem Cells International،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1213355
Modern Language Association (MLA)
Tasnim, Nishat…[et al.]. The Efficacy of Graphene Foams for Culturing Mesenchymal Stem Cells and Their Differentiation into Dopaminergic Neurons. Stem Cells International No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1213355
American Medical Association (AMA)
Tasnim, Nishat& Thakur, Vikram& Chattopadhyay, Munmun& Joddar, Binata. The Efficacy of Graphene Foams for Culturing Mesenchymal Stem Cells and Their Differentiation into Dopaminergic Neurons. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1213355
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213355