Derivation and Identification of Motor Neurons from Human Urine-Derived Induced Pluripotent Stem Cells
Joint Authors
Xie, Bingbing
Zhong, Xiufeng
Yi, Huan
Liu, Ben
Wang, Xuan
Xu, Li
Liu, Jia
Li, Min
Peng, Fuhua
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-01-24
Country of Publication
Egypt
No. of Pages
9
Abstract EN
Induced pluripotent stem cells (iPSCs) have provided new opportunities for motor neuron disease (MND) modeling, drug screening, and cellular therapeutic development.
Among the various types of iPSCs, urine-derived iPSCs have become a promising source of stem cells because they can be safely and noninvasively isolated and easily reprogrammed.
Here, for the first time, we differentiated urine-derived iPSCs (urine-iPSCs) into motor neurons (MNs) and compared the capacity of urine-iPSCs and cord-blood-derived iPSCs (B-iPSCs) to differentiate into MNs.
With the use of small molecules, mature MNs were generated from urine-iPSCs as early as 26 days in culture.
Furthermore, in coculture with muscle cells, MNs projected long axons and formed neuromuscular junctions (NMJs).
Immunofluorescence and PCR confirmed the expression levels of both MN and NMJ markers.
The comparison of the ratios of positive labeling for MN markers between urine-iPSCs and B-iPSCs demonstrated that the differentiation potentials of these cells were not significantly different.
The abovementioned results indicate that urine-iPSCs are a new, promising source of stem cells for MND modeling and further cellular therapeutic development.
American Psychological Association (APA)
Yi, Huan& Xie, Bingbing& Liu, Ben& Wang, Xuan& Xu, Li& Liu, Jia…[et al.]. 2018. Derivation and Identification of Motor Neurons from Human Urine-Derived Induced Pluripotent Stem Cells. Stem Cells International،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1213360
Modern Language Association (MLA)
Yi, Huan…[et al.]. Derivation and Identification of Motor Neurons from Human Urine-Derived Induced Pluripotent Stem Cells. Stem Cells International No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1213360
American Medical Association (AMA)
Yi, Huan& Xie, Bingbing& Liu, Ben& Wang, Xuan& Xu, Li& Liu, Jia…[et al.]. Derivation and Identification of Motor Neurons from Human Urine-Derived Induced Pluripotent Stem Cells. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1213360
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213360