Isolation and Characterization of Multipotent Turkey Tendon-Derived Stem Cells
Joint Authors
Zhao, Chunfeng
Liu, Qian
Zhu, Yaxi
Amadio, Peter C.
Moran, Steven L.
Gingery, Anne
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-06
Country of Publication
Egypt
No. of Pages
10
Abstract EN
Tendon injuries are among the most common and severe hand injuries with a high demand for functional recovery.
Stem cells have been identified and isolated from different species and a variety of tissues for the sake of regenerative medicine.
Recently, turkey has been suggested as a potential new large animal model for flexor tendon-related research.
However, turkey tissue-specific stem cells have not been investigated.
Here, we presented the isolation and verification of tendon-derived stem cells (TDSCs) from 6- to 8-month-old heritage-breed turkey.
TDSCs were isolated from turkey flexor tendon by plating nucleated cells at the determined optimal density.
Approximately 4% of the nucleated cells demonstrated clonogenicity, high proliferation rate, and trilineage differentiation potential after induction culturing.
These cells expressed surface antigens CD90, CD105, and CD44, but did not express CD45.
There was a high level of gene expression of tenogenic markers in TDSCs, including mohawk, collagen type I, tenascin C, and elastin.
Turkey TDSCs also expressed transcription factors PouV, Nanog, and Sox2, which are critically involved in the regulation of stemness.
The successful isolation of tendon-derived stem cells from turkey was beneficial for future studies in tendon tissue engineering and would help in the development of new treatment for tendon diseases using this novel animal model.
American Psychological Association (APA)
Liu, Qian& Zhu, Yaxi& Amadio, Peter C.& Moran, Steven L.& Gingery, Anne& Zhao, Chunfeng. 2018. Isolation and Characterization of Multipotent Turkey Tendon-Derived Stem Cells. Stem Cells International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1213362
Modern Language Association (MLA)
Liu, Qian…[et al.]. Isolation and Characterization of Multipotent Turkey Tendon-Derived Stem Cells. Stem Cells International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1213362
American Medical Association (AMA)
Liu, Qian& Zhu, Yaxi& Amadio, Peter C.& Moran, Steven L.& Gingery, Anne& Zhao, Chunfeng. Isolation and Characterization of Multipotent Turkey Tendon-Derived Stem Cells. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1213362
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213362