Developmental Pathways Pervade Stem Cell Responses to Evolving Extracellular Matrices of 3D Bioprinted Microenvironments
Joint Authors
Tran, Quyen A.
Ajeti, Visar
Freeman, Brian T.
Campagnola, Paul J.
Ogle, Brenda M.
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-29
Country of Publication
Egypt
No. of Pages
15
Abstract EN
Developmental studies and 3D in vitro model systems show that the production and engagement of extracellular matrix (ECM) often precede stem cell differentiation.
Yet, unclear is how the ECM triggers signaling events in sequence to accommodate multistep process characteristic of differentiation.
Here, we employ transcriptome profiling and advanced imaging to delineate the specificity of ECM engagement to particular differentiation pathways and to determine whether specificity in this context is a function of long-term ECM remodeling.
To this end, human mesenchymal stem cells (hMSCs) were cultured in 3D bioprinted prisms created from ECM proteins and associated controls.
We found that exogenous ECM provided in 3D microenvironments at early time points impacts on the composition of microenvironments at later time points and that each evolving 3D microenvironment is uniquely poised to promote stem cell differentiation.
Moreover, 2D cultures undergo minimal ECM remodeling and are ill-equipped to stimulate pathways associated with development.
American Psychological Association (APA)
Tran, Quyen A.& Ajeti, Visar& Freeman, Brian T.& Campagnola, Paul J.& Ogle, Brenda M.. 2018. Developmental Pathways Pervade Stem Cell Responses to Evolving Extracellular Matrices of 3D Bioprinted Microenvironments. Stem Cells International،Vol. 2018, no. 2018, pp.1-15.
https://search.emarefa.net/detail/BIM-1213433
Modern Language Association (MLA)
Tran, Quyen A.…[et al.]. Developmental Pathways Pervade Stem Cell Responses to Evolving Extracellular Matrices of 3D Bioprinted Microenvironments. Stem Cells International No. 2018 (2018), pp.1-15.
https://search.emarefa.net/detail/BIM-1213433
American Medical Association (AMA)
Tran, Quyen A.& Ajeti, Visar& Freeman, Brian T.& Campagnola, Paul J.& Ogle, Brenda M.. Developmental Pathways Pervade Stem Cell Responses to Evolving Extracellular Matrices of 3D Bioprinted Microenvironments. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-15.
https://search.emarefa.net/detail/BIM-1213433
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213433