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Human Recombinant Peptide Sponge Enables Novel, Less Invasive Cell Therapy for Ischemic Stroke
Joint Authors
Shichinohe, Hideo
Houkin, Kiyohiro
Kawabori, Masahito
Miyamoto, Michiyuki
Nakamura, Kentaro
Yamauchi, Tomohiro
Ito, Masaki
Saito, Hisayasu
Osanai, Toshiya
Sasaki, Tasuku
Kuroda, Satoshi
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-08
Country of Publication
Egypt
No. of Pages
8
Abstract EN
Bone marrow stromal cell (BMSC) transplantation has the therapeutic potential for ischemic stroke.
However, it is unclear which delivery routes would yield both safety and maximal therapeutic benefits.
We assessed whether a novel recombinant peptide (RCP) sponge, that resembles human collagen, could act as a less invasive and beneficial scaffold in cell therapy for ischemic stroke.
BMSCs from green fluorescent protein-transgenic rats were cultured and Sprague–Dawley rats were subjected to permanent middle cerebral artery occlusion (MCAo).
A BMSC-RCP sponge construct was transplanted onto the ipsilateral intact neocortex 7 days after MCAo.
A BMSC suspension or vehicle was transplanted into the ipsilateral striatum.
Rat motor function was serially evaluated and histological analysis was performed 5 weeks after transplantation.
The results showed that BMSCs could proliferate well in the RCP sponge and the BMSC-RCP sponge significantly promoted functional recovery, compared with the vehicle group.
Histological analysis revealed that the RCP sponge provoked few inflammatory reactions in the host brain.
Moreover, some BMSCs migrated to the peri-infarct area and differentiated into neurons in the BMSC-RCP sponge group.
These findings suggest that the RCP sponge may be a promising candidate for animal protein-free scaffolds in cell therapy for ischemic stroke in humans.
American Psychological Association (APA)
Miyamoto, Michiyuki& Nakamura, Kentaro& Shichinohe, Hideo& Yamauchi, Tomohiro& Ito, Masaki& Saito, Hisayasu…[et al.]. 2018. Human Recombinant Peptide Sponge Enables Novel, Less Invasive Cell Therapy for Ischemic Stroke. Stem Cells International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1213436
Modern Language Association (MLA)
Miyamoto, Michiyuki…[et al.]. Human Recombinant Peptide Sponge Enables Novel, Less Invasive Cell Therapy for Ischemic Stroke. Stem Cells International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1213436
American Medical Association (AMA)
Miyamoto, Michiyuki& Nakamura, Kentaro& Shichinohe, Hideo& Yamauchi, Tomohiro& Ito, Masaki& Saito, Hisayasu…[et al.]. Human Recombinant Peptide Sponge Enables Novel, Less Invasive Cell Therapy for Ischemic Stroke. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1213436
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213436