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The Effects of Hyperacute Serum on the Elements of the Human Subchondral Bone Marrow Niche
Joint Authors
Horváthy, Dénes B.
Kuten, Olga
Nehrer, Stefan
Simon, Melinda
Major, Bálint
Vácz, Gabriella
Hornyák, István
Hinsenkamp, Adél
Kardos, Dorottya
Bagó, Marcell
Cseh, Domonkos
Sárközi, Adrienn
Lacza, Zsombor
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-16
Country of Publication
Egypt
No. of Pages
12
Abstract EN
Mesenchymal stem cells (MSCs) are widely used in laboratory experiments as well as in human cell therapy.
Their culture requires animal sera like fetal calf serum (FCS) as essential supplementation; however, animal sera pose a risk for clinical applications.
Human blood derivatives, for example, platelet-rich plasma (PRP) releasates, are potential replacements of FCS; however, it is unclear which serum variant has the best effect on the given cell or tissue type.
Additionally, blood derivatives are commonly used in musculoskeletal diseases like osteoarthritis (OA) or osteonecrosis as “proliferative agents” for the topical MSC pool.
Hyperacute serum (HAS), a new serum derivative, has been designed to approximate the natural coagulation cascade with a single-step, additive-free preparation method.
We investigated the effects of HAS on monolayer MSC cultures and in their natural niche, in 3D subchondral bone and marrow explants.
Viability measurements, RT-qPCR evaluation for gene expression and flow cytometry for cell surface marker analysis were performed to compare the effects of FCS-, PRP-, or HAS-supplemented culture media.
Monolayer MSCs showed significantly higher metabolic activity following 5 days’ incubation in HAS, and osteoblast-specific mRNA expression was markedly increased, while cells also retained their MSC-specific cell surface markers.
A similar effect was observed on bone and marrow explants, which was further confirmed with confocal microscopy analysis.
Moreover, markedly higher bone marrow preservation was observed with histology in case of HAS supplementation compared to FCS.
These findings indicate possible application of HAS in regenerative solutions of skeletal diseases like OA or osteonecrosis.
American Psychological Association (APA)
Simon, Melinda& Major, Bálint& Vácz, Gabriella& Kuten, Olga& Hornyák, István& Hinsenkamp, Adél…[et al.]. 2018. The Effects of Hyperacute Serum on the Elements of the Human Subchondral Bone Marrow Niche. Stem Cells International،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1213447
Modern Language Association (MLA)
Simon, Melinda…[et al.]. The Effects of Hyperacute Serum on the Elements of the Human Subchondral Bone Marrow Niche. Stem Cells International No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1213447
American Medical Association (AMA)
Simon, Melinda& Major, Bálint& Vácz, Gabriella& Kuten, Olga& Hornyák, István& Hinsenkamp, Adél…[et al.]. The Effects of Hyperacute Serum on the Elements of the Human Subchondral Bone Marrow Niche. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1213447
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213447