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Evaluation of Oxidative Stress and Mitophagy during Adipogenic Differentiation of Adipose-Derived Stem Cells Isolated from Equine Metabolic Syndrome (EMS) Horses
Joint Authors
Kornicka, Katarzyna
Weiss, C.
Śmieszek, Agnieszka
Marycz, Krzysztof
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-06
Country of Publication
Egypt
No. of Pages
18
Abstract EN
Mesenchymal stem cells (MSCs) are frequently used in both human and veterinary medicine because their unique properties, such as modulating the immune response and differentiating into multiple lineages, make them a valuable tool in cell-based therapies.
However, many studies have indicated the age-, lifestyle-, and disease-related deterioration of MSC regenerative characteristics.
However, it still needs to be elucidated how the patient’s health status affects the effectiveness of MSC differentiation.
In the present study, we isolated mesenchymal stem cells from adipose tissue (adipose-derived mesenchymal stem cells (ASCs)) from horses diagnosed with equine metabolic syndrome (EMS), a common metabolic disorder characterized by pathological obesity and insulin resistance.
We investigated the metabolic status of isolated cells during adipogenic differentiation using multiple research methods, such as flow cytometry, PCR, immunofluorescence, or transmission and confocal microscopy.
The results indicated the impaired differentiation potential of ASCEMS.
Excessive ROS accumulation and ER stress are most likely the major factors limiting the multipotency of these cells.
However, we observed autophagic flux during differentiation as a protective mechanism that allows cells to maintain homeostasis and remove dysfunctional mitochondria.
American Psychological Association (APA)
Marycz, Krzysztof& Weiss, C.& Śmieszek, Agnieszka& Kornicka, Katarzyna. 2018. Evaluation of Oxidative Stress and Mitophagy during Adipogenic Differentiation of Adipose-Derived Stem Cells Isolated from Equine Metabolic Syndrome (EMS) Horses. Stem Cells International،Vol. 2018, no. 2018, pp.1-18.
https://search.emarefa.net/detail/BIM-1213464
Modern Language Association (MLA)
Marycz, Krzysztof…[et al.]. Evaluation of Oxidative Stress and Mitophagy during Adipogenic Differentiation of Adipose-Derived Stem Cells Isolated from Equine Metabolic Syndrome (EMS) Horses. Stem Cells International No. 2018 (2018), pp.1-18.
https://search.emarefa.net/detail/BIM-1213464
American Medical Association (AMA)
Marycz, Krzysztof& Weiss, C.& Śmieszek, Agnieszka& Kornicka, Katarzyna. Evaluation of Oxidative Stress and Mitophagy during Adipogenic Differentiation of Adipose-Derived Stem Cells Isolated from Equine Metabolic Syndrome (EMS) Horses. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-18.
https://search.emarefa.net/detail/BIM-1213464
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213464