Mesenchymal Stromal Cells Cultured in Serum from Heart Failure Patients Are More Resistant to Simulated Chronic and Acute Stress
Joint Authors
Falk, Volkmar
Nazari-Shafti, Timo Z.
Xu, Zhiyi
Bader, Andreas Matthäus
Henke, Georg
Klose, Kristin
Stamm, Ch.
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-01
Country of Publication
Egypt
No. of Pages
15
Abstract EN
Despite regulatory issues surrounding the use of animal-derived cell culture supplements, most clinical cardiac cell therapy trials using mesenchymal stromal cells (MSCs) still rely on fetal bovine serum (FBS) for cell expansion before transplantation.
We sought to investigate the effect of human serum from heart failure patients (HFS) on cord blood MSCs (CB-MSCs) during short-term culture under regular conditions and during simulated acute and chronic stress.
Cell survival, proliferation, metabolic activity, and apoptosis were quantified, and gene expression profiles of selected apoptosis and cell cycle regulators were determined.
Compared to FBS, HFS and serum from healthy donors (CS) showed similar effects by substantially increasing cell survival during chronic and acute stress and by increasing cell yields 5 days after acute stress.
Shortly after the termination of acute stress, both HFS and CS resulted in a marked decrease in apoptotic cells.
Transcriptome analysis suggested a decrease in TNF-mediated induction of caspases and decreased activation of mitochondrial apoptosis.
Our data confirm that human serum from both healthy donors and heart failure patients results in increased cell yields and increased resistance to cellular stress signals.
Therefore, we consider autologous serum a valid alternative to FBS in cell-based therapies addressing severe heart disease.
American Psychological Association (APA)
Nazari-Shafti, Timo Z.& Xu, Zhiyi& Bader, Andreas Matthäus& Henke, Georg& Klose, Kristin& Falk, Volkmar…[et al.]. 2018. Mesenchymal Stromal Cells Cultured in Serum from Heart Failure Patients Are More Resistant to Simulated Chronic and Acute Stress. Stem Cells International،Vol. 2018, no. 2018, pp.1-15.
https://search.emarefa.net/detail/BIM-1213483
Modern Language Association (MLA)
Nazari-Shafti, Timo Z.…[et al.]. Mesenchymal Stromal Cells Cultured in Serum from Heart Failure Patients Are More Resistant to Simulated Chronic and Acute Stress. Stem Cells International No. 2018 (2018), pp.1-15.
https://search.emarefa.net/detail/BIM-1213483
American Medical Association (AMA)
Nazari-Shafti, Timo Z.& Xu, Zhiyi& Bader, Andreas Matthäus& Henke, Georg& Klose, Kristin& Falk, Volkmar…[et al.]. Mesenchymal Stromal Cells Cultured in Serum from Heart Failure Patients Are More Resistant to Simulated Chronic and Acute Stress. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-15.
https://search.emarefa.net/detail/BIM-1213483
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213483