Ion Channel Expression and Characterization in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Joint Authors
El-Battrawy, Ibrahim
Akin, Ibrahim
Zhao, Zhihan
Lan, Huan
Li, Xin
Buljubasic, Fanis
Sattler, Katherine
Yücel, Gökhan
Lang, Siegfried
Tiburcy, Malte
Zimmermann, Wolfram-Hubertus
Cyganek, Lukas
Wieland, Thomas
Zhou, Xiao-Bo
Borggrefe, Martin
Utikal, Jochen
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-01-08
Country of Publication
Egypt
No. of Pages
14
Abstract EN
Background.
Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are providing new possibilities for the biological study, cell therapies, and drug discovery.
However, the ion channel expression and functions as well as regulations in hiPSC-CMs still need to be fully characterized.
Methods.
Cardiomyocytes were derived from hiPS cells that were generated from two healthy donors.
qPCR and patch clamp techniques were used for the study.
Results.
In addition to the reported ion channels, INa, ICa-L, ICa-T, If, INCX, IK1, Ito, IKr, IKs IKATP, IK-pH, ISK1–3, and ISK4, we detected both the expression and currents of ACh-activated (KACh) and Na+-activated (KNa) K+, volume-regulated and calcium-activated (Cl-Ca) Cl−, and TRPV channels.
All the detected ion currents except IK1, IKACh, ISK, IKNa, and TRPV1 currents contribute to AP duration.
Isoprenaline increased ICa-L, If, and IKs but reduced INa and INCX, without an effect on Ito, IK1, ISK1–3, IKATP, IKr, ISK4, IKNa, ICl-Ca, and ITRPV1.
Carbachol alone showed no effect on the tested ion channel currents.
Conclusion.
Our data demonstrate that most ion channels, which are present in healthy or diseased cardiomyocytes, exist in hiPSC-CMs.
Some of them contribute to action potential performance and are regulated by adrenergic stimulation.
American Psychological Association (APA)
Zhao, Zhihan& Lan, Huan& El-Battrawy, Ibrahim& Li, Xin& Buljubasic, Fanis& Sattler, Katherine…[et al.]. 2018. Ion Channel Expression and Characterization in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Stem Cells International،Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1213499
Modern Language Association (MLA)
Zhao, Zhihan…[et al.]. Ion Channel Expression and Characterization in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Stem Cells International No. 2018 (2018), pp.1-14.
https://search.emarefa.net/detail/BIM-1213499
American Medical Association (AMA)
Zhao, Zhihan& Lan, Huan& El-Battrawy, Ibrahim& Li, Xin& Buljubasic, Fanis& Sattler, Katherine…[et al.]. Ion Channel Expression and Characterization in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1213499
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213499