Human Bone Marrow Contains Mesenchymal Stromal Stem Cells That Differentiate In Vitro into Contractile Myofibroblasts Controlling T Lymphocyte Proliferation
Joint Authors
Lecarpentier, Yves
Schussler, Olivier
Sakic, Antonija
Rincon-Garriz, José Maria
Soulie, Priscilla
Bochaton-Piallat, Marie-Luce
Kindler, Vincent
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-29
Country of Publication
Egypt
No. of Pages
15
Abstract EN
Mesenchymal stromal stem cells (MSC) that reside in the bone marrow (BM) can be amplified in vitro.
In 2-dimension (D) cultures, MSC exhibit a morphology similar to fibroblasts, are able to inhibit T lymphocyte and natural killer cell proliferation, and can be differentiated into adipocytes, chondrocytes, or osteoblasts if exposed to specific media.
Here we show that medullar MSC cultured in 2D formed an adherent stroma of cells expressing well-organized microfilaments containing α-smooth muscle actin and nonmuscle myosin heavy chain IIA.
MSC could be grown in 3D in collagen membranes generating a structure which, upon exposition to 50 mM KCl or to an alternating electric current, developed a contractile strength that averaged 34 and 45 μN/mm2, respectively.
Such mechanical tension was similar in intensity and in duration to that of human placenta and was annihilated by isosorbide dinitrate or 2,3-butanedione monoxime.
Membranes devoid of MSC did not exhibit a significant contractility.
Moreover, MSC nested in collagen membranes were able to control T lymphocyte proliferation, and differentiated into adipocytes, chondrocytes, or osteoblasts.
Our observations show that BM-derived MSC cultured in collagen membranes spontaneously differentiate into contractile myofibroblasts exhibiting unexpected properties in terms of cell differentiation potential and of immunomodulatory function.
American Psychological Association (APA)
Lecarpentier, Yves& Schussler, Olivier& Sakic, Antonija& Rincon-Garriz, José Maria& Soulie, Priscilla& Bochaton-Piallat, Marie-Luce…[et al.]. 2018. Human Bone Marrow Contains Mesenchymal Stromal Stem Cells That Differentiate In Vitro into Contractile Myofibroblasts Controlling T Lymphocyte Proliferation. Stem Cells International،Vol. 2018, no. 2018, pp.1-15.
https://search.emarefa.net/detail/BIM-1213505
Modern Language Association (MLA)
Lecarpentier, Yves…[et al.]. Human Bone Marrow Contains Mesenchymal Stromal Stem Cells That Differentiate In Vitro into Contractile Myofibroblasts Controlling T Lymphocyte Proliferation. Stem Cells International No. 2018 (2018), pp.1-15.
https://search.emarefa.net/detail/BIM-1213505
American Medical Association (AMA)
Lecarpentier, Yves& Schussler, Olivier& Sakic, Antonija& Rincon-Garriz, José Maria& Soulie, Priscilla& Bochaton-Piallat, Marie-Luce…[et al.]. Human Bone Marrow Contains Mesenchymal Stromal Stem Cells That Differentiate In Vitro into Contractile Myofibroblasts Controlling T Lymphocyte Proliferation. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-15.
https://search.emarefa.net/detail/BIM-1213505
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213505