Transplantation of Human Amniotic Membrane over the Liver Surface Reduces Hepatic Fibrosis in a Cholestatic Model in Young Rats
Joint Authors
Parraga, Mario
San Martin, S.
Cordova, C.
Garrido, M.
Escobar, C.
Zamora, C.
Rejas, C.
Varas, J.
Papuzinski, C.
Montedonico, S.
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-02-25
Country of Publication
Egypt
No. of Pages
9
Abstract EN
Purpose. Biliary atresia precedes liver cirrhosis and liver transplantation.
Amniotic membrane (AM) promotes tissue regeneration, inhibits fibrosis, and reduces inflammation.
Here, we test amniotic membrane potential as a therapeutic tool against cholestatic liver fibrosis.
Methods.
Three groups of rats were used: sham surgery (SS), bile duct ligature (BDL), and bile duct ligature plus human amniotic membrane (BDL + AM).
After surgery, animals were sacrificed at different weeks.
Biochemical and histopathological analyses of liver tissue were performed.
Collagen was expressed as a percentage of total liver tissue area.
qPCR was performed to analyse gene expression levels of transforming growth factor-β1 (Tgfb1) and apelin (Apln).
Statistical analysis performed considered p<0.05 was significant.
Results. Groups undergoing BDL developed cholestasis.
Biochemical markers from BDL + AM group improved compared to BDL group.
Ductular reaction, portal fibrosis, and bile plugs were markedly reduced in the BDL + AM group compared to BDL group.
Collagen area in BDL + AM group was statistically decreased compared to BDL group.
Finally, expression levels of both Apln and Tgfb1 mRNA were statistically downregulated in BDL + AM group versus BDL group.
Conclusion.
AM significantly reduces liver fibrosis in a surgical animal model of cholestasis.
Our results suggest that AM may be useful as a therapeutic tool in liver cirrhosis.
American Psychological Association (APA)
Garrido, M.& Escobar, C.& Zamora, C.& Rejas, C.& Varas, J.& Cordova, C.…[et al.]. 2018. Transplantation of Human Amniotic Membrane over the Liver Surface Reduces Hepatic Fibrosis in a Cholestatic Model in Young Rats. Stem Cells International،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1213508
Modern Language Association (MLA)
Garrido, M.…[et al.]. Transplantation of Human Amniotic Membrane over the Liver Surface Reduces Hepatic Fibrosis in a Cholestatic Model in Young Rats. Stem Cells International No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1213508
American Medical Association (AMA)
Garrido, M.& Escobar, C.& Zamora, C.& Rejas, C.& Varas, J.& Cordova, C.…[et al.]. Transplantation of Human Amniotic Membrane over the Liver Surface Reduces Hepatic Fibrosis in a Cholestatic Model in Young Rats. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1213508
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213508