![](/images/graphics-bg.png)
Altered Expression of Hematopoiesis Regulatory Molecules in Lipopolysaccharide-Induced Bone Marrow Mesenchymal Stem Cells of Patients with Aplastic Anemia
Joint Authors
Tripathy, Naresh Kumar
Nityanand, Soniya
Chaturvedi, Chandra Prakash
Minocha, Ekta
Sharma, Akhilesh
Rahman, Khaliqur
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-10-17
Country of Publication
Egypt
No. of Pages
7
Abstract EN
We have investigated the expression of RNA transcripts of hematopoiesis regulatory molecules, viz., macrophage inflammatory protein (MIP)-1α, tumor necrosis factor (TNF)-α, granulocyte colony-stimulating factor (G-CSF), stromal cell-derived factor (SDF)-1α, stem cell factor (SCF), and transforming growth factor (TGF)-β in lipopolysaccharide-induced bone marrow mesenchymal stem cells (BM-MSCs) and levels of their soluble forms in the culture supernatants of BM-MSCs and BM plasma of patients with acquired aplastic anemia (AA) (n=29) and controls (n=29).
The BM-MSCs of AA patients as compared to controls had markedly lower expression of MIP-1α transcripts (p<0.001), higher expression of TNF-α (p<0.001), G-CSF (p<0.001), and SDF-1α (p<0.01) transcripts, and no difference in the expression of SCF and TGF-β transcripts.
The culture supernatants of BM-MSCs and BM plasma of AA patients in comparison to controls also had lower levels of MIP-1α (p<0.01 and p<0.001, respectively) and higher levels of TNF-α (p<0.05 for both) and G-CSF (p<0.05 and p<0.001, respectively) but with no difference in the levels of SDF-1α and SCF.
The levels of TGF-β were although similar in culture supernatants of BM-MSCs of both the groups, but they were significantly lower in BM plasma of the patients than controls (p<0.001).
Our data shows that BM-MSCs of AA patients have altered expression of hematopoiesis regulatory molecules suggesting that they may have a role in the pathogenesis of the disease.
American Psychological Association (APA)
Chaturvedi, Chandra Prakash& Tripathy, Naresh Kumar& Minocha, Ekta& Sharma, Akhilesh& Rahman, Khaliqur& Nityanand, Soniya. 2018. Altered Expression of Hematopoiesis Regulatory Molecules in Lipopolysaccharide-Induced Bone Marrow Mesenchymal Stem Cells of Patients with Aplastic Anemia. Stem Cells International،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1213529
Modern Language Association (MLA)
Chaturvedi, Chandra Prakash…[et al.]. Altered Expression of Hematopoiesis Regulatory Molecules in Lipopolysaccharide-Induced Bone Marrow Mesenchymal Stem Cells of Patients with Aplastic Anemia. Stem Cells International No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1213529
American Medical Association (AMA)
Chaturvedi, Chandra Prakash& Tripathy, Naresh Kumar& Minocha, Ekta& Sharma, Akhilesh& Rahman, Khaliqur& Nityanand, Soniya. Altered Expression of Hematopoiesis Regulatory Molecules in Lipopolysaccharide-Induced Bone Marrow Mesenchymal Stem Cells of Patients with Aplastic Anemia. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1213529
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213529