Novel Calcium Phosphate Cement with Metformin-Loaded Chitosan for Odontogenic Differentiation of Human Dental Pulp Cells
Joint Authors
Ma, Tao
Xu, Hockin H. K.
Weir, Michael D.
Qin, Wei
Lin, Zhengmei
Chen, Jia-Yao
Guo, Jia
Guo, Dong
Shu, Yan
Schneider, Abraham
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-27
Country of Publication
Egypt
No. of Pages
10
Abstract EN
Metformin is an old and widely accepted first-line drug for treating type 2 diabetes.
Our previous studies demonstrate that metformin can stimulate the osteo/odontogenic differentiation of human-induced pluripotent stem cell-derived mesenchymal stem cells and human dental pulp cells (DPCs).
Due to the rapid dilution of metformin from the defect area, the aim of this study was to develop a drug delivery system with controlled release of metformin to promote cell viability and odontogenic differentiation of DPCs favoring dentin regeneration.
Calcium phosphate cement (CPC) containing chitosan and metformin as a scaffold was synthesized.
DPCs were seeded onto the scaffold, and the viability and proliferation were evaluated at several time points.
For osteogenic differentiation analysis, alkaline phosphatase (ALP) activity was tested, cells were stained with Alizarin Red, and the expression of odontogenic markers was evaluated by real-time polymerase chain reaction.
DPCs remained viable and attached well to the CPC-chitosan composite scaffold.
Moreover, the addition of metformin to the CPC-chitosan composite did not adversely affect cell proliferation, compared to that of CPC control.
Our data further revealed that the novel CPC-chitosan-metformin composite enhanced the odontogenic differentiation of DPCs, as evidenced by higher ALP activity, elevated expression of odontoblastic markers, and strong mineral deposition.
These results suggest that the new CPC-chitosan-metformin composite is a highly promising scaffold with the potential for tissue engineering applications including dentin regeneration.
American Psychological Association (APA)
Qin, Wei& Chen, Jia-Yao& Guo, Jia& Ma, Tao& Weir, Michael D.& Guo, Dong…[et al.]. 2018. Novel Calcium Phosphate Cement with Metformin-Loaded Chitosan for Odontogenic Differentiation of Human Dental Pulp Cells. Stem Cells International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1213552
Modern Language Association (MLA)
Qin, Wei…[et al.]. Novel Calcium Phosphate Cement with Metformin-Loaded Chitosan for Odontogenic Differentiation of Human Dental Pulp Cells. Stem Cells International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1213552
American Medical Association (AMA)
Qin, Wei& Chen, Jia-Yao& Guo, Jia& Ma, Tao& Weir, Michael D.& Guo, Dong…[et al.]. Novel Calcium Phosphate Cement with Metformin-Loaded Chitosan for Odontogenic Differentiation of Human Dental Pulp Cells. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1213552
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213552