Angiotensin II Attenuates the Bioactivities of Human Endothelial Progenitor Cells via Downregulation of β2-Adrenergic Receptor

Joint Authors

Yun, Jisoo
Kwon, Sang-Mo
Ji, Seung Taek
Kim, Yeon-Ju
Park, Ji Hye
Jang, Woong Bi
Kim, Da Yeon
Kang, Songhwa
Seong, Ha Jong
Lee, Dong Hyung
Jung, Seok Yun
Lee, Seon Jin
Choi, Sung Hyun
Lee, Dongjun

Source

Stem Cells International

Issue

Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2018-10-29

Country of Publication

Egypt

No. of Pages

11

Abstract EN

Cross talks between the renin-angiotensin system (RAS), sympathetic nervous system, and vascular homeostasis are tightly coordinated in hypertension.

Angiotensin II (Ang II), a key factor in RAS, when abnormally activated, affects the number and bioactivity of circulating human endothelial progenitor cells (hEPCs) in hypertensive patients.

In this study, we investigated how the augmentation of Ang II regulates adrenergic receptor-mediated signaling and angiogenic bioactivities of hEPCs.

Interestingly, the short-term treatment of hEPCs with Ang II drastically attenuated the expression of beta-2 adrenergic receptor (ADRB2), but did not alter the expression of beta-1 adrenergic receptor (ADRB1) and Ang II type 1 receptor (AT1R).

EPC functional assay clearly demonstrated that the treatment with ADRB2 agonists significantly increased EPC bioactivities including cell proliferation, migration, and tube formation abilities.

However, EPC bioactivities were decreased dramatically when treated with Ang II.

Importantly, the attenuation of EPC bioactivities by Ang II was restored by treatment with an AT1R antagonist (telmisartan; TERT).

We found that AT1R binds to ADRB2 in physiological conditions, but this binding is significantly decreased in the presence of Ang II.

Furthermore, TERT, an Ang II-AT1R interaction blocker, restored the interaction between AT1R and ADRB2, suggesting that Ang II might induce the dysfunction of EPCs via downregulation of ADRB2, and an AT1R blocker could prevent Ang II-mediated ADRB2 depletion in EPCs.

Taken together, our report provides novel insights into potential therapeutic approaches for hypertension-related cardiovascular diseases.

American Psychological Association (APA)

Lee, Seon Jin& Kim, Da Yeon& Yun, Jisoo& Choi, Sung Hyun& Jung, Seok Yun& Kang, Songhwa…[et al.]. 2018. Angiotensin II Attenuates the Bioactivities of Human Endothelial Progenitor Cells via Downregulation of β2-Adrenergic Receptor. Stem Cells International،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1213563

Modern Language Association (MLA)

Lee, Seon Jin…[et al.]. Angiotensin II Attenuates the Bioactivities of Human Endothelial Progenitor Cells via Downregulation of β2-Adrenergic Receptor. Stem Cells International No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1213563

American Medical Association (AMA)

Lee, Seon Jin& Kim, Da Yeon& Yun, Jisoo& Choi, Sung Hyun& Jung, Seok Yun& Kang, Songhwa…[et al.]. Angiotensin II Attenuates the Bioactivities of Human Endothelial Progenitor Cells via Downregulation of β2-Adrenergic Receptor. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1213563

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1213563