Pulsed Electromagnetic Fields Improve Tenogenic Commitment of Umbilical Cord-Derived Mesenchymal Stem Cells: A Potential Strategy for Tendon Repair—An In Vitro Study
Joint Authors
Marmotti, A.
Mattia, S.
Blonna, D.
Peretti, G. M.
De Girolamo, Laura
Setti, Stefania
Mangiavini, L.
Viganò, Marco
Ferrero, Giovanni
Castoldi, Filippo
Bonasia, Davide Edoardo
Bellato, Enrico
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-30
Country of Publication
Egypt
No. of Pages
18
Abstract EN
Tendon repair is a challenging procedure in orthopaedics.
The use of mesenchymal stem cells (MSCs) and pulsed electromagnetic fields (PEMF) in tendon regeneration is still investigational.
In this perspective, MSCs isolated from the human umbilical cord (UC) may represent a possible candidate for tendon tissue engineering.
The aim of the study is to evaluate the effect of low-frequency PEMF on tenogenic differentiation of MSCs isolated from the human umbilical cord (UC-MSCs) in vitro.
15 fresh UC samples from women with healthy pregnancies were retrieved at the end of caesarean deliveries.
UC samples were manually minced into small fragments (less than 4 mm length) and cultured in MSC expansion medium.
Part of the UC-MSCs was subsequently cultured with PEMF and tenogenic growth factors.
UC-MSCs were subjected to pulsed electromagnetic fields for 2 h/day, 4 h/day, or 8 h/day.
UC-MSCs cultured with FGF-2 and stimulated with PEMF showed a greater production of collagen type I and scleraxis.
The prolonged exposure to PEMF was also related to the greatest expression of tenogenic markers.
Thus, the exposure to PEMF provides a positive preconditioning biophysical stimulus, which may enhance UC-MSC tenogenic potential.
American Psychological Association (APA)
Marmotti, A.& Peretti, G. M.& Mattia, S.& Mangiavini, L.& De Girolamo, Laura& Viganò, Marco…[et al.]. 2018. Pulsed Electromagnetic Fields Improve Tenogenic Commitment of Umbilical Cord-Derived Mesenchymal Stem Cells: A Potential Strategy for Tendon Repair—An In Vitro Study. Stem Cells International،Vol. 2018, no. 2018, pp.1-18.
https://search.emarefa.net/detail/BIM-1213631
Modern Language Association (MLA)
Marmotti, A.…[et al.]. Pulsed Electromagnetic Fields Improve Tenogenic Commitment of Umbilical Cord-Derived Mesenchymal Stem Cells: A Potential Strategy for Tendon Repair—An In Vitro Study. Stem Cells International No. 2018 (2018), pp.1-18.
https://search.emarefa.net/detail/BIM-1213631
American Medical Association (AMA)
Marmotti, A.& Peretti, G. M.& Mattia, S.& Mangiavini, L.& De Girolamo, Laura& Viganò, Marco…[et al.]. Pulsed Electromagnetic Fields Improve Tenogenic Commitment of Umbilical Cord-Derived Mesenchymal Stem Cells: A Potential Strategy for Tendon Repair—An In Vitro Study. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-18.
https://search.emarefa.net/detail/BIM-1213631
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213631