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IGF-1-Overexpressing Mesenchymal StemStromal Cells Promote Immunomodulatory and Proregenerative Effects in Chronic Experimental Chagas Disease
Joint Authors
Soares, Milena Botelho
Meira, Cássio Santana
Ribeiro-dos-Santos, Ricardo
Souza, Bruno Solano de Freitas
Silva, Daniela Nascimento
Azevedo, Carine Machado
Carvalho, Gisele Batista
Vasconcelos, Juliana Fraga
de Jesus, Paloma G.
Feitoza, Malena S.
Cavalcante, Bruno Raphael
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-24
Country of Publication
Egypt
No. of Pages
11
Abstract EN
Mesenchymal stem/stromal cells (MSCs) have been investigated for the treatment of diseases that affect the cardiovascular system, including Chagas disease.
MSCs are able to promote their beneficial actions through the secretion of proregenerative and immunomodulatory factors, including insulin-like growth factor-1 (IGF-1), which has proregenerative actions in the heart and skeletal muscle.
Here, we evaluated the therapeutic potential of IGF-1-overexpressing MSCs (MSC_IGF-1) in a mouse model of chronic Chagas disease.
C57BL/6 mice were infected with Colombian strain Trypanosoma cruzi and treated with MSCs, MSC_IGF-1, or vehicle (saline) six months after infection.
RT-qPCR analysis confirmed the presence of transplanted cells in both the heart and skeletal muscle tissues.
Transplantation of either MSCs or MSC_IGF-1 reduced the number of inflammatory cells in the heart when compared to saline controls.
Moreover, treatment with MSCs or MSC_IGF-1 significantly reduced TNF-α, but only MSC treatment reduced IFN-γ production compared to the saline group.
Skeletal muscle sections of both MSC- and MSC_IGF-1-treated mice showed a reduction in fibrosis compared to saline controls.
Importantly, the myofiber area was reduced in T.
cruzi-infected mice, and this was recovered after treatment with MSC_IGF-1.
Gene expression analysis in the skeletal muscle showed a higher expression of pro- and anti-inflammatory molecules in MSC_IGF-1-treated mice compared to MSCs alone, which significantly reduced the expression of TNF-α and IL-1β.
In conclusion, our results indicate the therapeutic potential of MSC_IGF-1, with combined immunomodulatory and proregenerative actions to the cardiac and skeletal muscles.
American Psychological Association (APA)
Silva, Daniela Nascimento& Souza, Bruno Solano de Freitas& Azevedo, Carine Machado& Vasconcelos, Juliana Fraga& de Jesus, Paloma G.& Feitoza, Malena S.…[et al.]. 2018. IGF-1-Overexpressing Mesenchymal StemStromal Cells Promote Immunomodulatory and Proregenerative Effects in Chronic Experimental Chagas Disease. Stem Cells International،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1213636
Modern Language Association (MLA)
Silva, Daniela Nascimento…[et al.]. IGF-1-Overexpressing Mesenchymal StemStromal Cells Promote Immunomodulatory and Proregenerative Effects in Chronic Experimental Chagas Disease. Stem Cells International No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1213636
American Medical Association (AMA)
Silva, Daniela Nascimento& Souza, Bruno Solano de Freitas& Azevedo, Carine Machado& Vasconcelos, Juliana Fraga& de Jesus, Paloma G.& Feitoza, Malena S.…[et al.]. IGF-1-Overexpressing Mesenchymal StemStromal Cells Promote Immunomodulatory and Proregenerative Effects in Chronic Experimental Chagas Disease. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1213636
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213636