Oxygen Tension Strongly Influences Metabolic Parameters and the Release of Interleukin-6 of Human Amniotic Mesenchymal Stromal Cells In Vitro
Joint Authors
Steinborn, Ralf
Weidinger, Adelheid
Dumitrescu, Sergiu
Kozlov, Andrey V.
Banerjee, Asmita
Lindenmair, Andrea
Wolbank, Susanne
Redl, Heinz
Hennerbichler, Simone
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-10-28
Country of Publication
Egypt
No. of Pages
11
Abstract EN
The human amniotic membrane (hAM) has been used for tissue regeneration for over a century.
In vivo (in utero), cells of the hAM are exposed to low oxygen tension (1–4% oxygen), while the hAM is usually cultured in atmospheric, meaning high, oxygen tension (20% oxygen).
We tested the influence of oxygen tensions on mitochondrial and inflammatory parameters of human amniotic mesenchymal stromal cells (hAMSCs).
Freshly isolated hAMSCs were incubated for 4 days at 5% and 20% oxygen.
We found 20% oxygen to strongly increase mitochondrial oxidative phosphorylation, especially in placental amniotic cells.
Oxygen tension did not impact levels of reactive oxygen species (ROS); however, placental amniotic cells showed lower levels of ROS, independent of oxygen tension.
In contrast, the release of nitric oxide was independent of the amniotic region but dependent on oxygen tension.
Furthermore, IL-6 was significantly increased at 20% oxygen.
To conclude, short-time cultivation at 20% oxygen of freshly isolated hAMSCs induced significant changes in mitochondrial function and release of IL-6.
Depending on the therapeutic purpose, cultivation conditions of the cells should be chosen carefully for providing the best possible quality of cell therapy.
American Psychological Association (APA)
Banerjee, Asmita& Lindenmair, Andrea& Steinborn, Ralf& Dumitrescu, Sergiu& Hennerbichler, Simone& Kozlov, Andrey V.…[et al.]. 2018. Oxygen Tension Strongly Influences Metabolic Parameters and the Release of Interleukin-6 of Human Amniotic Mesenchymal Stromal Cells In Vitro. Stem Cells International،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1213694
Modern Language Association (MLA)
Banerjee, Asmita…[et al.]. Oxygen Tension Strongly Influences Metabolic Parameters and the Release of Interleukin-6 of Human Amniotic Mesenchymal Stromal Cells In Vitro. Stem Cells International No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1213694
American Medical Association (AMA)
Banerjee, Asmita& Lindenmair, Andrea& Steinborn, Ralf& Dumitrescu, Sergiu& Hennerbichler, Simone& Kozlov, Andrey V.…[et al.]. Oxygen Tension Strongly Influences Metabolic Parameters and the Release of Interleukin-6 of Human Amniotic Mesenchymal Stromal Cells In Vitro. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1213694
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213694