User-Friendly Genetic Conditional Knockout Strategies by CRISPRCas9
Joint Authors
Wu, Qiang
Zhang, Wensheng
Chen, Liangliang
Ye, Ying
Dai, Hongxia
Zhang, Heyao
Zhang, Xue
Zhu, Zhexin
Spalinskas, Rapolas
Ren, Wenyan
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-14
Country of Publication
Egypt
No. of Pages
10
Abstract EN
Loss-of-function studies are critically important in gene functional analysis of model organisms and cells.
However, conditional gene inactivation in diploid cells is difficult to achieve, as it involves laborious vector construction, multifold electroporation, and complicated genotyping.
Here, a strategy is presented for generating biallelic conditional gene and DNA regulatory region knockouts in mouse embryonic stem cells by codelivery of CRISPR-Cas9 and short-homology-arm targeting vectors sequentially or simultaneously.
Collectively, a simple and rapid method was presented to knock out any DNA element conditionally.
This approach will facilitate the functional studies of essential genes and regulatory regions during development.
American Psychological Association (APA)
Chen, Liangliang& Ye, Ying& Dai, Hongxia& Zhang, Heyao& Zhang, Xue& Wu, Qiang…[et al.]. 2018. User-Friendly Genetic Conditional Knockout Strategies by CRISPRCas9. Stem Cells International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1213697
Modern Language Association (MLA)
Chen, Liangliang…[et al.]. User-Friendly Genetic Conditional Knockout Strategies by CRISPRCas9. Stem Cells International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1213697
American Medical Association (AMA)
Chen, Liangliang& Ye, Ying& Dai, Hongxia& Zhang, Heyao& Zhang, Xue& Wu, Qiang…[et al.]. User-Friendly Genetic Conditional Knockout Strategies by CRISPRCas9. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1213697
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213697