The Alterations of Biofilm Formation and EPS Characteristics of a Diatom by a Sponge-Associated Bacterium Psychrobacter sp.
Joint Authors
Zhou, Xiaojian
Meng, Jie
Yu, Zhaowei
Miao, Li
Jin, Cuili
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-24
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
A sponge-associated bacterium, which was identified as Psychrobacter sp.
in this study, was found with high activity against biofilm formation of benthic diatoms, including Amphora sp., Nitzschia closterium, Nitzschia frustulum, and Stauroneis sp.
The activity against diatom biofilm formation by the tested strain was confirmed mostly in the culture supernatant and could be extracted using organic solvents.
Treatment with its supernatant crude extract significantly reduced the cells of Stauroneis sp.
forming biofilm and slightly increased the cells floating in the culture medium, which results in the ratio of biofilm cell/floating cell altering from 0.736 in control to 0.414 in treatment.
Use of the supernatant crude extract led to increased production of extracellular polymeric substances (EPSs) by diatom Stauroneis sp.
from 16.66 to 41.59 (g/g cell dry weight).
The increase in EPS production was mainly contributed by soluble EPS (SL-EPS) and followed by the EPS that was tightly bound to biofilm cells (BF-TB-EPS).
In addition, the supernatant crude extract caused significant changes in the monosaccharides composition of the EPS of Stauroneis sp.
Specifically, glucuronic acid (Glc-A) and N-acetyl-D-glucosamine (Glc-NAc) in BF-TB-EPS were 55% fold decreased and 1219% fold increased, respectively.
Based on our findings, we proposed that these changes in monosaccharides composition might lead to a decreased biofilm formation efficiency of diatom.
American Psychological Association (APA)
Zhou, Xiaojian& Meng, Jie& Yu, Zhaowei& Miao, Li& Jin, Cuili. 2018. The Alterations of Biofilm Formation and EPS Characteristics of a Diatom by a Sponge-Associated Bacterium Psychrobacter sp.. Scientifica،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1213756
Modern Language Association (MLA)
Zhou, Xiaojian…[et al.]. The Alterations of Biofilm Formation and EPS Characteristics of a Diatom by a Sponge-Associated Bacterium Psychrobacter sp.. Scientifica No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1213756
American Medical Association (AMA)
Zhou, Xiaojian& Meng, Jie& Yu, Zhaowei& Miao, Li& Jin, Cuili. The Alterations of Biofilm Formation and EPS Characteristics of a Diatom by a Sponge-Associated Bacterium Psychrobacter sp.. Scientifica. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1213756
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213756