Hydrolysis Activity of Virgin Coconut Oil Using Lipase from Different Sources
Joint Authors
Nguyen, Van Thi Ai
Le, Truong Dang
Phan, Hoa Ngoc
Tran, Lam Bich
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-01-14
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Two types of lipase, Candida rugosa lipase (CRL) and porcine pancreas lipase (PPL), were used to hydrolyze virgin coconut oil (VCO).
The hydrolysis process was carried out under four parameters, VCO to buffer ratio, lipase concentration, pH, and temperature, which have a significant effect on hydrolysis of lipase.
CRL obtained the best hydrolysis condition at 1 : 5 of VCO to buffer ratio, 1.5% of CRL concentration, pH 7, and temperature of 40°C.
Meanwhile, PPL gave different results at 1 : 4 of VCO to buffer ratio, 2% of lipase concentration, pH 7.5, and 40°C.
The highest hydrolysis degree of CRL and PPL was obtained after 16 hours and 26 hours, reaching 79.64% and 27.94%, respectively.
Besides, the hydrolysis process was controlled at different time course (every half an hour) at the first 4 hours of reaction to compare the initial hydrolysis degree of these two lipase types.
FFAs from hydrolyzed products were isolated and determined the percentage of each fatty acid which contributes to the FFAs mixture.
As a result, medium chain fatty acids (MCFAs) made up the main contribution in composition of FFAs and lauric acid (C12) was the largest segment (47.23% for CRL and 44.23% for PPL).
American Psychological Association (APA)
Nguyen, Van Thi Ai& Le, Truong Dang& Phan, Hoa Ngoc& Tran, Lam Bich. 2018. Hydrolysis Activity of Virgin Coconut Oil Using Lipase from Different Sources. Scientifica،Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1213789
Modern Language Association (MLA)
Nguyen, Van Thi Ai…[et al.]. Hydrolysis Activity of Virgin Coconut Oil Using Lipase from Different Sources. Scientifica No. 2018 (2018), pp.1-6.
https://search.emarefa.net/detail/BIM-1213789
American Medical Association (AMA)
Nguyen, Van Thi Ai& Le, Truong Dang& Phan, Hoa Ngoc& Tran, Lam Bich. Hydrolysis Activity of Virgin Coconut Oil Using Lipase from Different Sources. Scientifica. 2018. Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1213789
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213789