In Vitro Micropropagation of Industrially and Medicinally Useful Plant Aloe trichosantha Berger Using Offshoot Cuttings
Joint Authors
Berhe Sbhatu, Desta
Abraha, Haftom Baraki
Hailu, Atsbeha
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-16
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
This study was aimed to develop in vitro micropropagation protocol of Aloe trichosantha Berger using offshoots as explants.
MS media supplemented with plant growth regulators helped explants develop shoots within about 14 to 17 days.
The mean number of days to shooting has decreased from 16.8 ± 0.8 with 0.5/0.5 mg/L BAP/NAA supplement to 15.5 ± 0.5 with 2.0/0.5 mg/L BAP/NAA.
While the mean shoot number has increased with increasing the concentration of BAP supplements, the reverse was true with mean shoot lengths, whereas supplement of 2.0/0.5 mg/L BAP/NAA has generated significantly more shoots (17 ± 3.8), and longer shoots were produced with the addition of 0.5/0.5 and 1.0/0.5 mg/L BAP/NAA.
In regard to rooting, though higher concentrations of NAA have resulted in quick rooting, the rooting performance in terms of mean number and length of roots was better with low concentrations.
All the plantlets subjected to greenhouse acclimatization in cocopeat have survived.
Secondary acclimatization in composted and manured soil media has also resulted in 93 to 95% survival rate.
Lighting conditions (nursery shade or direct sunlight) of secondary acclimatization did not lead to any difference in the survival rate of the plantlets.
American Psychological Association (APA)
Hailu, Atsbeha& Berhe Sbhatu, Desta& Abraha, Haftom Baraki. 2020. In Vitro Micropropagation of Industrially and Medicinally Useful Plant Aloe trichosantha Berger Using Offshoot Cuttings. The Scientific World Journal،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1213873
Modern Language Association (MLA)
Hailu, Atsbeha…[et al.]. In Vitro Micropropagation of Industrially and Medicinally Useful Plant Aloe trichosantha Berger Using Offshoot Cuttings. The Scientific World Journal No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1213873
American Medical Association (AMA)
Hailu, Atsbeha& Berhe Sbhatu, Desta& Abraha, Haftom Baraki. In Vitro Micropropagation of Industrially and Medicinally Useful Plant Aloe trichosantha Berger Using Offshoot Cuttings. The Scientific World Journal. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1213873
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213873