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Determination of Cassiarin A Level of Cassia siamea Leaf Obtained from Various Regions in Indonesia Using the TLC-Densitometry Method
Joint Authors
Ekasari, Wiwied
Widyawaruyanti, Aty
Widiyastuti, Yuli
Subositi, Dyah
Hamsidi, Rini
Basuki, Sukmawati
Setyawan, Dwi
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-07
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Cassia siamea leaf has been proven in vitro and in vivo to have a strong antimalarial activity with Cassiarin A as its active compound.
To obtain a source of C.
siamea medicinal plant with high level of active antimalarial compound (Cassiarin A), a valid method for determining Cassiarin A level is needed.
For this reason, this research conducts the validation of the Cassiarin A content with determination method using thin-layer chromatography (TLC) densitometry which includes the determination of selectivity (Rs), linearity (r), accuracy, precision, limit of detection (LOD), and limit of quantification (LOQ).
Cassiarin A was chromatographed on silica gel 60 F254 TLC plate using chloroform : ethanol (85 : 15 v/v) as a mobile phase.
Cassiarin A was quantified by densitometric analysis at 368 nm.
The linear regression analysis data for the calibration plots showed good linear relationship with r = 0.9995.
The method was validated for precision, recovery, repeatability.
The minimum detectable amount was found to be 0.0027 μg/spot, whereas the limit of quantitation was found to be 0.008 μg/spot.
The results of this validation are then used to determine the Cassiarin A level of C.
siamea leaf from various regions in Indonesia.
Based on the results of the study, it can be concluded that the TLC-densitometry method can be used to determine level of the Cassiarin A compound with the advantages of being fast, easy, accurate, and inexpensive.
In addition, it showed that C.
siamea leaves from Pacitan have the highest level of Cassiarin A compared to other areas studied.
American Psychological Association (APA)
Ekasari, Wiwied& Widiyastuti, Yuli& Subositi, Dyah& Hamsidi, Rini& Widyawaruyanti, Aty& Basuki, Sukmawati…[et al.]. 2020. Determination of Cassiarin A Level of Cassia siamea Leaf Obtained from Various Regions in Indonesia Using the TLC-Densitometry Method. The Scientific World Journal،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1214013
Modern Language Association (MLA)
Ekasari, Wiwied…[et al.]. Determination of Cassiarin A Level of Cassia siamea Leaf Obtained from Various Regions in Indonesia Using the TLC-Densitometry Method. The Scientific World Journal No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1214013
American Medical Association (AMA)
Ekasari, Wiwied& Widiyastuti, Yuli& Subositi, Dyah& Hamsidi, Rini& Widyawaruyanti, Aty& Basuki, Sukmawati…[et al.]. Determination of Cassiarin A Level of Cassia siamea Leaf Obtained from Various Regions in Indonesia Using the TLC-Densitometry Method. The Scientific World Journal. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1214013
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1214013