Phylogenetic Analysis and Antibiotics Resistance of Listeria Monocytogenes Contaminating Chicken Meat in Surabaya, Indonesia
Joint Authors
Pertiwi, Herinda
Aksono, Eduardus Bimo
Riwu, Katty Hendriana Priscilla
Estoepangestie, A. T. Soelih
Source
Veterinary Medicine International
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-03-01
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The objective of this study was to identify the phylogenetic analysis and antibiotic resistance of Listeria monocytogenes contaminating chicken meat in Surabaya.
60 chicken meat samples were collected from supermarkets, mobile vendors, and traditional markets in Surabaya.
A selective medium is used for isolation and identification of Listeria monocytogenes by chopping 25 grams of the chicken meat and to put it into the sterilized Erlenmeyer flasks.
Some methods were used for the identification procedures, such as biochemical and morphological tests, antibiotic resistance test, PCR, and sequencing; also a phylogenetic analysis was conducted by a neighbor-joining analysis using Genetix Mac ver 8.0 with hlyA genes of Listeria monocytogenes recorded in GenBank, such as Lineage I (KC808543), Lineage II (AY229462, AY229346, AY229499, and AY229404), Lineage III (KJ504139, HQ686043, KJ504116, and DQ988349), and Lineage IV (EU840690, EF030606).
The result shows that the prevalence of L.
monocytogenes in Surabaya contaminating the chicken meat samples from the supermarkets was 10% (2/20), from the mobile vendors was 0/20 (0%), and from the traditional markets was 5% (1/20).
It was seen from the band at 456 bp fragment.
Furthermore, three isolates found in Surabaya were included in the new lineages which were resistant to old-generation antibiotics such as sulfamethonazole-trimetophrim (SXT) and amoxyllin sulbactam (MAS), but they were still sensitive to new-generation antibiotics such as cefotaxime (CTX) and meropenem (MEM).
American Psychological Association (APA)
Aksono, Eduardus Bimo& Riwu, Katty Hendriana Priscilla& Estoepangestie, A. T. Soelih& Pertiwi, Herinda. 2020. Phylogenetic Analysis and Antibiotics Resistance of Listeria Monocytogenes Contaminating Chicken Meat in Surabaya, Indonesia. Veterinary Medicine International،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1214288
Modern Language Association (MLA)
Aksono, Eduardus Bimo…[et al.]. Phylogenetic Analysis and Antibiotics Resistance of Listeria Monocytogenes Contaminating Chicken Meat in Surabaya, Indonesia. Veterinary Medicine International No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1214288
American Medical Association (AMA)
Aksono, Eduardus Bimo& Riwu, Katty Hendriana Priscilla& Estoepangestie, A. T. Soelih& Pertiwi, Herinda. Phylogenetic Analysis and Antibiotics Resistance of Listeria Monocytogenes Contaminating Chicken Meat in Surabaya, Indonesia. Veterinary Medicine International. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1214288
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1214288