فعالية خلاصات نبات الشيح الحولي المضادة لبعض الجراثيم الممرضة متعددة المقاومة

Abstract EN

Artemisia annua is a medicinal and Syrian plant belonging to the Asteraceae, recommended by the World Health Organization (WHO) as an antimalarial agent.

With the emergence of multi-drug resistant bacterial strains, as well as the scarcity of local research, this research aims to study the antibacterial activity of the extracts of this plant against some pathogenic multidrug resistant bacteria.

The agar well diffusion method was used to study the antibacterial activity of extracts.

Micro-dilution method was used to determine the minimal inhibitory concentration.

Disk diffusion method was used to study antibacterial resistance to antibiotics.

Standard antibiotic disks were infused with plant extracts to study synergistic effects.

The yield of plant extracts ranged between 1.62% for leaf hexane extract and 27.64% for flowering aerial parts aqueous extract.

The most resistant bacteria was the first isolate of Pseudomonas aeruginosa (P.

a1), which was resistant to nine antibiotics belonging to seven groups, the least resistant bacteria was the second isolation of staphylococcus aureus (S.

a2), which was resistant to seven antibiotics belonging to four groups.

methanol extract was the most potent and The broader spectrum, Where the diameter of inhibition zones were 12.8, 14, 10.66, 13.33, 17.66 and 19.33 mm for Escherichia coli (E.

c1), Escherichia coli (E.

c2), Pseudomonas aeruginosa (P.

a1), Pseudomonas aeruginosa (P.

a2), Staphylococcus aureus (S.

a1) and Staphylococcus aureus (S.

a2), respectively, when using 75 ^l of leaf methanolic extract.

The minimal inhibitory concentration (MIC) ranged from 0.39 mg/ml of leaf and flowering aerial parts hexane extract for Staphylococcus aureus.

and 25 mg/ml of leaf aqueous extract for Escherichia coli and Pseudomonas aeruginosa.

Synergy between some antibiotics and some plant extracts has occurred, e.g., the diameter inhibition zone for Staphylococcus aureus (S.

a2) was increased from 12 mm when the use of amikacin (AK) to 22 mm when using the amikacin disk mixed with methanol extract.

It was concluded that the methanol extract can be used as anti-multidrug resistant bacteria, especially staphylococcus aureus.