تحديد الأنواع الوراثية [P] و G لفيروس روتا الإنسان في أطفال محافظة بابل باستخدام تقنية تفاعل البلمرة المتسلسل التقليدي PCR-conventional

Abstract EN

Rapid test device technique and conventional - PCR technique was used in this study for detection of rotavirus genotypes in stool samples which were collected from children who were living in different area of Babylon governorate with symptoms of acute gastroenteritis such as diarrhoea , fever and vomition for the period from October 2011 to March of 2012 , Their age range from 0 – 60 months , 470 diarrhoea stools sample from children was tested by using rapid stripe , then 80 sample which gave positive result by rapid test device were used to know G and [P] type by using specific G and [P] primer .After extraction of viral RNA and its convertion to cDNA .

The results detected that the prevalent G – genotypes was G1 , G4 , G2 and G8 respectively , whereas P[8] , P[4] and P[6] was the most dispersal P – genotypes