Micropropagation and genetic variations of Cordyline terminalis cv. red top

Abstract EN

Due to the slow, expensive and time - consumed conventional propagation of Cordyline , and the merits of in vitro propagation for overcoming on these problems.

The present study was undertaken.

Simple protocol for in vitro regeneration of Cordyline terminalis cv.

Red Top using shoot tip explants was achieved.

For initiation stage , shoot tip explants were cultured on MS medium fortified with both 3% sucrose (w/v) and 0.2% gelrite (w/v).

As for multiplication stage, the new formed axillary shoots were cultured on MS fortified with various concentrations of benzyladenine (BA; 0,1, 3, 5 and 7 mg/l) and thidiazuron (TDZ; 0, 0.5 ,1, 1.5 and 2 mg/l) in the presence of 1 mg/l Kinetin (Kin) were used.

As for shoot elongation and in vitro rooting stage, new formed shoots derived from multiplication stage were culture on MSPGRs -free medium gelled with 2% (w/v)gelrite .

Concerning the acclimatization stage, the rhizogened plantlets were potted in mixture media consists of peat moss and vermiculite (1:1 %).

The survival percentage of new formed plantlets after acclimatization was 100 % of the treatments; 1 mg/l BAP +1 mg/l Kin, 7 mg/l BAP +1 mg/l Kin and 1.5 mg/l TDZ +1 mg/l Kin under the light intensity 30 μmol m-2 s-1.

The procedure outlined has fulfilled the requirements for micropropagation of Cordyline.

Randomly amplified polymorphic DNA (RAPD) analysis showed that the polymorphism level was the same at all primers (100%)