Evaluation of microbial community in wastewater treatment plant using qRT-PCR protocol
Other Title(s)
تقييم الحمل الميكروبى في محطة معالجة مياه الصرف الصحي باستخدام تقنية qRT-PCR
Joint Authors
Majdi, Mahmud
al-Atiq, Sawsan Yusuf
Sabri, Abanub Tharwat
Source
Arab Universities Journal of Agricultural Sciences
Issue
Vol. 28, Issue 4 (31 Dec. 2020), pp.1159-1166, 8 p.
Publisher
Ain Shams University Faculty of Agriculture
Publication Date
2020-12-31
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Topics
- Activities
- Water treatment
- Middle East
- Molecular genetics
- Water treatment plants
- Polymerase chain reaction
- Linear models(Statistics)
Abstract EN
Recycling treated wastewater is considered one of the most critical sectors in recycling.
Its benefit fell beyond the disposability of sewage flow that extends to the possibility of reusing the water.
Wastewater treatment in any system depends exclusively on maximizing the activity of the microbial community.
Wastewater samples were collected from two systems under the same administration in one of the largest wastewater treatment plants (WWTPs) in the Middle East (Al-Jabal Al-Asfar).
Five major collection points for the daily assessment of the plant were used for collecting samples.
In this study, real-time quantification polymerase chain reaction (qRT-PCR) was used to quantify the microbial community to evaluate the two treatment systems in the plant.
Based on the results, the microbial community in each of the five major points from both systems was quantified based on the linear model produced from qRT-PCR.
One of the two systems was active and efficient, in contrast to the other, which lacked profound control over the treatment.
The current study suggests the routine usage of qRT-PCR as a crucial molecular genetics technique to evaluate the microbial community within the wastewater to ensure reliable and efficient treatment of the raw sewage.
The study recommends the full assessment of the inactive system within the plant, encouraging the usage of compatible microbes to enrich and activate the returned activated sludge (RAS) for better performance.
American Psychological Association (APA)
al-Atiq, Sawsan Yusuf& Sabri, Abanub Tharwat& Majdi, Mahmud. 2020. Evaluation of microbial community in wastewater treatment plant using qRT-PCR protocol. Arab Universities Journal of Agricultural Sciences،Vol. 28, no. 4, pp.1159-1166.
https://search.emarefa.net/detail/BIM-1240542
Modern Language Association (MLA)
al-Atiq, Sawsan Yusuf…[et al.]. Evaluation of microbial community in wastewater treatment plant using qRT-PCR protocol. Arab Universities Journal of Agricultural Sciences Vol. 28, no. 4 (2020), pp.1159-1166.
https://search.emarefa.net/detail/BIM-1240542
American Medical Association (AMA)
al-Atiq, Sawsan Yusuf& Sabri, Abanub Tharwat& Majdi, Mahmud. Evaluation of microbial community in wastewater treatment plant using qRT-PCR protocol. Arab Universities Journal of Agricultural Sciences. 2020. Vol. 28, no. 4, pp.1159-1166.
https://search.emarefa.net/detail/BIM-1240542
Data Type
Journal Articles
Language
English
Notes
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Record ID
BIM-1240542