p53, PTEN tumor suppressor gene methylation in thyroid cancer
Joint Authors
Ahmad, Ghazwan Fawzi
Pity, Intisar Salim
Salih, Ahmad Muhammad
Source
al-Kufa University Journal for Biology
Issue
Vol. 11, Issue 3 (31 Dec. 2019), pp.8-20, 13 p.
Publisher
University of Kufa Faculty of Science Department of live Sciences
Publication Date
2019-12-31
Country of Publication
Iraq
No. of Pages
13
Main Subjects
Topics
Abstract EN
Background and objective: DNA methylation serves as an alternative to the mutational inactivation due to epigenetic event that alters gene expression without changing its DNA sequence.
Such alterations may silence putative DNA repair genes and tumor suppressor genes whose role is undisputed in malignant transformation.
This study was performed to detect the frequency of p53 and PTEN methylation in different thyroid cancers.
Method: Using methylation specific PCR methods, DNA was extracted from 76 surgically resected thyroid tissues with primary cancer.
The target (p53, PTEN tumor suppressor genes) DNA was amplified with the mutation-specific primers.
Results: p53 gene complete methylation and partial methylation were 13.2%, and 17.1% respectively, whereas PTEN complete methylation and partial methylation were 18.4% and 23.7% respectively.
Complete p53 methylation was significantly frequent among Anaplastic thyroid cancer (ATC) cases (50.0%) compared with other thyroid cancer, followed by Follicular thyroid cancer (FTC) (33.3%).
Papillary carcinoma cases were more likely to be unmethylated for p53 gene.
Despite the trend of negatively associated PTEN complete methylation with any of the studied clinicopathological parameters, however, merging partial with complete methylation gave a significant association of PTEN methylation with FTC and, inversely, there was low link with Papillary thyroid cancer (PTC).
Conclusion: Combined silencing of PTEN and p53 faithfully reproduces the development of ATC, and may provide a compelling rationale for a future target chemotherapy.
p53 methylation appears low among early staged (T1, T2 and N0) tumors may indicate that p53 silencing is a hallmark of advanced tumors and so has bad ominous sign.
PTEN methylation is also significantly associated with follicular carcinoma whereas, inversely, low in papillary carcinoma.
American Psychological Association (APA)
Ahmad, Ghazwan Fawzi& Pity, Intisar Salim& Salih, Ahmad Muhammad. 2019. p53, PTEN tumor suppressor gene methylation in thyroid cancer. al-Kufa University Journal for Biology،Vol. 11, no. 3, pp.8-20.
https://search.emarefa.net/detail/BIM-1251908
Modern Language Association (MLA)
Ahmad, Ghazwan Fawzi…[et al.]. p53, PTEN tumor suppressor gene methylation in thyroid cancer. al-Kufa University Journal for Biology Vol. 11, no. 3 (2019), pp.8-20.
https://search.emarefa.net/detail/BIM-1251908
American Medical Association (AMA)
Ahmad, Ghazwan Fawzi& Pity, Intisar Salim& Salih, Ahmad Muhammad. p53, PTEN tumor suppressor gene methylation in thyroid cancer. al-Kufa University Journal for Biology. 2019. Vol. 11, no. 3, pp.8-20.
https://search.emarefa.net/detail/BIM-1251908
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 18-20
Record ID
BIM-1251908