Green silver nanoparticles production by and against the two-spotted spider mite Tetranychus urticae (Acari : Tetranychidae)‎

Abstract EN

The novel method to produce green silver nanoparticles by Tetranychus urticae Koch.

( Acari: Tetranychidae) was depicted to provide new biocides.

It was started by the exposure of adult females of T.

urticae to little amounts of silver nitrate AgNO3.Then, table-salt, sodium chloride, was used as an intermediate compound to produce biological spherical silver nanoparticles (AgNPs) by mites.

Coating with biogenic amines released from exploded T.urticae was detected by monoamine oxidase interaction.

Then, such a structure played an important role to penetrate the integument of exposed T.urticae easily.

Bionanoparticles of silver produced from the green form of T.urticae, which caused certain mortality percentages over 97% against the same species, while they were more than 90% against the red form of T.urticae.

In the same trend, the bio product from the red form of T.urticae, caused mortality over 88% and 94% in the case of treatments against green and red T.urticae forms, respectively.

Consequently, determined LC50s of bio AgNPs from green against both forms were 20.58 and 31.81 μLL¯¹ while they were 29.24 and 58.35 μLL¯¹ of bio AgNPs from red T.urticae and against the same arrangement of morphs.

Therefore, AgNPs resulted from the green form of T.urticae, caused sterility with 91.21% and 86.07 % in case of treatments against T.urticae green and red forms, respectively.

Also, AgNPs from the red form of T.urticae, caused sterility with 88.68% and 94.21 % in case of treatments against the same arrangement of morphs, in comparison with control.

Revealed data showed that oxidative stress interacted effectively by increase with induced treatments.

Reactive oxygen scavengers (ROS) were significantly lower than control (P < 0.05).

To conclude, the new trend to produce bio AgNPs by mite against the same pest presented a cheap, simple and ecofriendly method to expand the use of nanoparticles in the plant protection field.