Molecular characterization of staphylococcus species isolated from clinical and subclinical mastitic buffaloes in el-Behera governorate, Egypt \
Author
Source
Alexandria Journal of Veterinary Sciences
Issue
Vol. 69, Issue 1 (30 Apr. 2021), pp.30-32, 3 p.
Publisher
Alexandria University Faculty of Veterinary Medicine
Publication Date
2021-04-30
Country of Publication
Egypt
No. of Pages
3
Main Subjects
Abstract EN
In the present study, a total of 120 milk samples (50 clinical and 70 subclinical mastitis cases) collected from buffaloes located at different localities at El-Behera Governorate, Egypt were examined phenotypically and genotypically for the presence of S.
aureus and coagulase-negative staphylococci.
Results showed that 42 S.
aureus and 20 coagulase-negative Staphylococcus species isolates were recovered from the examined 120 milk samples.
A total of 40 S.
aureus and coagulase-negative staphylococci were further identified based on the amplification of 16S rRNA, coa, clfA, and nuc encoding genes.
Results revealed that all S.
aureus isolated from clinical and subclinical cases were found to harbor 16S rRNA, coa, clfA, and nuc encoding genes.
Resistance against 12 antibiotics was determined using disc diffusion methods.
All the tested S.
aureus and coagulase-negative staphylococcal isolates exhibited resistance to amoxicillin, ampicillin, cefotaxime, ciprofloxacin, cloxacillin, oxacillin, lincomycin, penicillin, and trimethoprim/sulphamethoxazole.
Constitutive production of mecA and blaZ encoding resistance against penicillin and cloxacillin was reported in 70% and 20% of S.
aureus and 80 % and 30% of the coagulase-negative staphylococcal isolated from clinical and subclinical mastitis, respectively.
In contrast, all isolates found to harbor the specific amplicon of the sul1 gene.
The amplicon of aac(6”)-aph(2”) encoding resistance gene was observed in only 6 S.
aureus isolates.
The specific amplicon of norA gene was detected in 4 S.
aureus and 2 coagulase-negative staphylococcal isolates recovered from clinical mastitis.
To conclude, the emergence of multi-drug resistant S.
aureus and coagulase-negative staphylococci as a cause of mastitis in dairy farms is of public health concern requires further investigations.
American Psychological Association (APA)
Tarabis, Rida. 2021. Molecular characterization of staphylococcus species isolated from clinical and subclinical mastitic buffaloes in el-Behera governorate, Egypt \. Alexandria Journal of Veterinary Sciences،Vol. 69, no. 1, pp.30-32.
https://search.emarefa.net/detail/BIM-1254007
Modern Language Association (MLA)
Tarabis, Rida. Molecular characterization of staphylococcus species isolated from clinical and subclinical mastitic buffaloes in el-Behera governorate, Egypt \. Alexandria Journal of Veterinary Sciences Vol. 69, no. 1 (Apr. 2021), pp.30-32.
https://search.emarefa.net/detail/BIM-1254007
American Medical Association (AMA)
Tarabis, Rida. Molecular characterization of staphylococcus species isolated from clinical and subclinical mastitic buffaloes in el-Behera governorate, Egypt \. Alexandria Journal of Veterinary Sciences. 2021. Vol. 69, no. 1, pp.30-32.
https://search.emarefa.net/detail/BIM-1254007
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 29-32
Record ID
BIM-1254007