Ameliorative effects of rhamnus fruit (Ziziphus spina-christi L.)‎ and zinc on sodium fluoride-induced oxidative stress in rats

Abstract EN

Background: Fluoride is widely distributed in nature in many forms and its compounds are being used extensively.

Increased oxidative stress is proposed to mediate the toxic effects of fluoride on soft tissues.

Anti-inflammatory and antioxidant properties have been describedrom species of the Rhamnaceae family.

Aim of the study: Accordingly, this research was conducted to investigate the possible protective effects of rhamnus fruit Ziziphus spina-christi L.

 (Powder and extract), zinc and their co-treatment against sodium fluoride (NaF)-induced oxidative stress in male albino rats.

Materials and methods: Thirty five rats were divided into 7 equal groups.

Group 1 served as negative control group fed on the basal diet.

Group 2: positive control group was given NaF (10 mg/kg) orally once daily for 2 weeks.

Group 3: zinc 20mg/Kg b.

wt/ rats.

Group 4: fed on the formulated diet with 5% rhamnus fruit powder.

Group 5: given orally rhamnus extract at dose of 5 ml.

Group 6: 5% rhamnus fruit powder +zinc and Group 7: 5 ml of rhamnusfruit extract +zinc.

At the end of the experiment, all animals were sacrificed and blood samples were obtained for assessment of serum total cholesterol, HDL-C, LDL-C, VLDL-C and triglycerides levels in addition to liver and kidney functions.

Oxidative indices including total antioxidant status (TAS), total oxidant status (TOS), kidney TAS and TOS.

As well as, kidneys histopathological changes were assessed.

Results: NaF intoxicated groups showed significant alterations of biochemical indices with significantly decreased in TAS and TOS levels.

Conclusions: The obtained results showed that NaF intoxication caused hepatic and renal damage by increasing oxidative stress and suggesteda possible protective effect of rhamnus fruits and zinc administration against fluoride-induced oxidative stress.