The antioxidant effect of jumbolan ethanolic extract seeds against carbon tetra chloride induced kidney toxicity in experimental rats

Abstract EN

The present work investigated the effects of Syzgium cumini L seeds ethanolic extract against carbon tetrachloride induced kidney toxicity in Sprague–Dawley male rats.

Twenty four adult male albino rats were divided into two main groups: The first main group (6 rats) fed on basal diet as control negative group (C-ve).

The second main group (18 rats) fed on basal diet for 28 days.

At 27^th and 28^th days all rats were administrated ccl4 by gavage (1ml/kgb.

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The second main group was divided into 3 groups each group contained 6 rats as follows: Group 1: positive control, rats fed on basal diet only (C+ve).

Group 2&3: fed on basal diet and received jumbolan seeds extract (250 and 500 mgkg) orally.

At the end of the experiment biological data were calculated, blood samples were taken, kidney collected, weighted.

Serum was separated to biochemical analysis.

Tissue lipid peroxidation (LPX) and activity of antioxidant enzymes in kidney were also performed.

Also histopathological examination for kidneys were performed.

Results showed that CCl4 caused elevation in serum levels of creatinine, urea, uric acid, serum potassium, sodium, glucose and liver function.

MDA level was also increased significantly whereas SOD, and CAT levels were decreased in the kidney tissue homogenates of CCl4 treated rats.

Ethanolic extract of jumbolan seeds successfully improve the alterations of these effects in the experimental animals.

Our study demonstrated that the ethanolic extract of jumbolan seeds could protect kidney tissues against CCl4-induced oxidative stress by increasing ant oxidative defense activities.