The fitness of a gem strain of pseudomonas aeruginosabacteria comparing with its parent strains under laboratory conditions
Other Title(s)
كفاءة سلالة مهندسة وراثيا من بكتيريا Pseudomonas aeruginosa مقارنة بالسلالات الأبوية تحت الظروف المعملية
Joint Authors
al-Ashtukhi, Muhammad Mamduh Abd al-Tawwab
Amin, Mamduh Kamil Ali
Hasan, Ahmad Shawqi
Mati, Salah al-Sayid
Source
Zagazig Journal for Agricultural Researches
Issue
Vol. 48, Issue 2 (31 Mar. 2021), pp.469-480, 12 p.
Publisher
Zagazig University Faculty of Agriculture
Publication Date
2021-03-31
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Topics
Abstract EN
The fitnessof a genetically engineered microorganism strainthat constructed as transconjugant by conjugation mechanism of horizontal gene transfer in Pseudomonas aeruginosa bacteria comparing with its parent strains has been assessed.
Ecological fitness expressesinteractionof an organism with its environment.
This consider as a good indicator for the assessment of Genetically Engineered Microorganism (GEM).
When released into the environment this assay was carried out under laboratory condition to investigate the effects of environmental condition on the fitness.
These laboratory condition included the effect of differentcations(Na+, Ca+2, and Fe+3), different incubation temperatures, pH values and different ratios of the two parents.
Increasing of mono, and di cations (Na+, Ca+2) concentrations revealed an increasing in the fitness of parents and transconjugant (GEM( strain up to the maximum concentration (105 mM) with Na+ and (85mM) with Ca+2 and then began to decrease.
In the three strains temperature plays an important role in gene transfer rate and fitness, There was a direct relationship between the increasing in temperature until it reaches the optimum temperature and the rate of gene transfer.
Also, the highest rate of gene transfer via conjugation appeared when the ratio between the donor and the recipient was 3: 1.
The best results for bacterial fitness for transconjugant strain and parentsappeared at pH 9, which means increased fitness for both of them.
These results show that the transconjugant strain can be have and survive well as its parent strain and the effects of some ecological factor are similar in the three strains.
American Psychological Association (APA)
Mati, Salah al-Sayid& Hasan, Ahmad Shawqi& Amin, Mamduh Kamil Ali& al-Ashtukhi, Muhammad Mamduh Abd al-Tawwab. 2021. The fitness of a gem strain of pseudomonas aeruginosabacteria comparing with its parent strains under laboratory conditions. Zagazig Journal for Agricultural Researches،Vol. 48, no. 2, pp.469-480.
https://search.emarefa.net/detail/BIM-1255445
Modern Language Association (MLA)
Mati, Salah al-Sayid…[et al.]. The fitness of a gem strain of pseudomonas aeruginosabacteria comparing with its parent strains under laboratory conditions. Zagazig Journal for Agricultural Researches Vol. 48, no. 2 (2021), pp.469-480.
https://search.emarefa.net/detail/BIM-1255445
American Medical Association (AMA)
Mati, Salah al-Sayid& Hasan, Ahmad Shawqi& Amin, Mamduh Kamil Ali& al-Ashtukhi, Muhammad Mamduh Abd al-Tawwab. The fitness of a gem strain of pseudomonas aeruginosabacteria comparing with its parent strains under laboratory conditions. Zagazig Journal for Agricultural Researches. 2021. Vol. 48, no. 2, pp.469-480.
https://search.emarefa.net/detail/BIM-1255445
Data Type
Journal Articles
Language
English
Notes
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Record ID
BIM-1255445