Sera level and polymorphism of interleukin-33 gene in iraqi females patients with breast cancer
Joint Authors
al-Abbasi, Hazimah Musa
Abd al-Karim, Zainab Jalil
al-Sayid, Maha Muhammad
Muhaidi, Asma M. Salih
Source
Ibn al-Haitham Journal for Pure and Applied Science
Issue
Vol. 34, Issue 1 (31 Mar. 2021), pp.1-11, 11 p.
Publisher
University of Baghdad College of Education for Pure Science / Ibn al-Haitham
Publication Date
2021-03-31
Country of Publication
Iraq
No. of Pages
11
Main Subjects
Topics
Abstract EN
Interleukin-33 [IL-33] is a specific ligand for the ST2 receptor, and a member of theIL-1 family.
It is a dual-function protein that acts both as an extracellular alarmin cytokine, and an as an intracellular nuclear factor participates in maintaining barrier function byregulating gene expression of IL-33 modulating tumor growth and anti-tumor immunity incancer patients.
The present study aimed to investigate the role of IL-33 serum level and genepolymorphism in Iraqi women with breast cancer.
Materials and methods: Blood sampleswere collected from 66 Iraqi patient women diagnosed with breast cancer, which were dividedinto two groups: pre-treatment [PT] and under treatment with chemotherapy [UTC] patients inaddition to 34 apparently healthy women who were matched with patients as a control.
ELISA technique was used to determine the IL-33 serum level.
The PCR-RFLP techniquewas performed to determine IL-33 gene polymorphisms at single nucleotide polymorphismSNP [rs1929992].
Results: IL-33 serum level recorded lower significant in PT [182.22 ±29.86pg/ml] and UTC [129.87 ± 45.11pg/ml] patients compared with control [258.08 ± 39.54pg/ml] under [p<0.05].
In a polymorphism study on IL-33 SNP [rs1929992] showed AGgenotype recorded the high frequency in control than in patients [76.47, 58.46] compared toother genotypes AA, GG with no significant difference according to fisher's exact probability.
In conclusion: IL-33 serum level decreased after performing surgery on breast cancerpatients.
There was no association between breast cancer development and IL-33 SNP[rs1929992].
The heterozygous genotype AG was a common genotype in the Iraqi population.
Allele G had an environmental fraction, while allele A had preventive fraction, which related to increasing the level of IL-33 with both genotypes AA, AG in the healthy control group, incontrast with GG genotype which showed the highest level in the patients' is a specific ligand for the ST2 receptor, and a member of theIL-1 family.
It is a dual-function protein that acts both as an extracellular alarmin cytokine, and an as an intracellular nuclear factor participates in maintaining barrier function byregulating gene expression of IL-33 modulating tumor growth and anti-tumor immunity incancer patients.
The present study aimed to investigate the role of IL-33 serum level and genepolymorphism in Iraqi women with breast cancer.
Materials and methods: Blood sampleswere collected from 66 Iraqi patient women diagnosed with breast cancer, which were dividedinto two groups: pre-treatment [PT] and under treatment with chemotherapy [UTC] patients inaddition to 34 apparently healthy women who were matched with patients as a control.
ELISA technique was used to determine the IL-33 serum level.
The PCR-RFLP techniquewas performed to determine IL-33 gene polymorphisms at single nucleotide polymorphismSNP [rs1929992].
Results: IL-33 serum level recorded lower significant in PT [182.22 ±29.86pg/ml] and UTC [129.87 ± 45.11pg/ml] patients compared with control [258.08 ± 39.54pg/ml] under [p<0.05].
In a polymorphism study on IL-33 SNP [rs1929992] showed AGgenotype recorded the high frequency in control than in patients [76.47, 58.46] compared toother genotypes AA, GG with no significant difference according to fisher's exact probability.
In conclusion: IL-33 serum level decreased after performing surgery on breast cancerpatients.
There was no association between breast cancer development and IL-33 SNP[rs1929992].
The heterozygous genotype AG was a common genotype in the Iraqi population.
Allele G had an environmental fraction, while allele A had preventive fraction, which related to increasing the level of IL-33 with both genotypes AA, AG in the healthy control group, incontrast with GG genotype which showed the highest level in the patients' group
American Psychological Association (APA)
Muhaidi, Asma M. Salih& al-Abbasi, Hazimah Musa& Abd al-Karim, Zainab Jalil& al-Sayid, Maha Muhammad. 2021. Sera level and polymorphism of interleukin-33 gene in iraqi females patients with breast cancer. Ibn al-Haitham Journal for Pure and Applied Science،Vol. 34, no. 1, pp.1-11.
https://search.emarefa.net/detail/BIM-1255705
Modern Language Association (MLA)
al-Abbasi, Hazimah Musa…[et al.]. Sera level and polymorphism of interleukin-33 gene in iraqi females patients with breast cancer. Ibn al-Haitham Journal for Pure and Applied Science Vol. 34, no. 1 (2021), pp.1-11.
https://search.emarefa.net/detail/BIM-1255705
American Medical Association (AMA)
Muhaidi, Asma M. Salih& al-Abbasi, Hazimah Musa& Abd al-Karim, Zainab Jalil& al-Sayid, Maha Muhammad. Sera level and polymorphism of interleukin-33 gene in iraqi females patients with breast cancer. Ibn al-Haitham Journal for Pure and Applied Science. 2021. Vol. 34, no. 1, pp.1-11.
https://search.emarefa.net/detail/BIM-1255705
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 9-11
Record ID
BIM-1255705