Morphology and molecular studies of Contracaecum larvae (nematoda : anisakidae) in some fish species from Sulaimani province, Kurdistan region, Iraq
Other Title(s)
دراسة مظهرية و جزيئية ليرقة الديدان الخيطية Contracaecum في بعض أنواع الأسماك في محافظة السليمانية أقليم كوردستان، العراق
Joint Authors
Husayn, Rida Hasan
Abd Allah, Yunus Sabir
Abd Allah, Shamal Muhammad Amin
Source
Basrah Journal of Agrricultural Science
Issue
Vol. 34, Issue 1 (30 Jun. 2021), pp.93-110, 18 p.
Publisher
University of Basrah Agriculture College
Publication Date
2021-06-30
Country of Publication
Iraq
No. of Pages
18
Main Subjects
Topics
Abstract EN
Contracaecum is a genus of nematodes belonging to the family Anisakidae, that parasitise many fishes which act as an intermediate or paratenic hosts, while the piscivorous birds and mammals are definitive hosts.
A total of 44 third larval stage of Contracaecum were collected from 13 infected freshwater fishes belonging to five different species in different water bodies in Sulaimani Province, Kurdistan Region, Iraq from January to the end of December 2018.
In this investigation, 966 fishes were collected including six species of Nemacheilidae, five species of Cyprinidae, three species of Leuciscidae, two species of Xenocyprididae, one species of each of Bagridae, Heteropneustidae, Mastacembelidae, Mugilidae, Siluridae and Sisoridae.
This study revealed that five fish species (Cyprinus carpio, Luciobarbus barbulus, L.
esocinus, L.
xanthopterus and Mastacembelus mastacembelus) were infected with Contracaecum larvae with the prevalence of 2.05 %, 0.92 %, 1.92 %, 19.35 % and 1.06 %, respectively.
The Contracaecum larvae were morphologically studied by compound light microscope and the molecular analyses was done by amplification, sequencing and comparing different gene loci (ITS1, ITS2 and COX2) of isolated third larval stage of Contracaecum.
The ITS1, ITS2 and COX2 were amplified by polymerase chain reaction (PCR) and sequenced.
The sequences of ITS1, ITS2 and COX2 reveal that all Contracaecum larvae from all infected fishes represented exactly one species (Contracaecum rudolphii B) based on compering and identity percentage in Gene Bank database.
Phylogenetic analysis of the genotype (for ITS1) was described.
The genetic characterization of the Contracaecum larvae in the present study is available in the GenBank database and they were deposited in GenBank and their accession numbers were demonstrated.
American Psychological Association (APA)
Abd Allah, Yunus Sabir& Abd Allah, Shamal Muhammad Amin& Husayn, Rida Hasan. 2021. Morphology and molecular studies of Contracaecum larvae (nematoda : anisakidae) in some fish species from Sulaimani province, Kurdistan region, Iraq. Basrah Journal of Agrricultural Science،Vol. 34, no. 1, pp.93-110.
https://search.emarefa.net/detail/BIM-1258835
Modern Language Association (MLA)
Abd Allah, Yunus Sabir…[et al.]. Morphology and molecular studies of Contracaecum larvae (nematoda : anisakidae) in some fish species from Sulaimani province, Kurdistan region, Iraq. Basrah Journal of Agrricultural Science Vol. 34, no. 1 (2021), pp.93-110.
https://search.emarefa.net/detail/BIM-1258835
American Medical Association (AMA)
Abd Allah, Yunus Sabir& Abd Allah, Shamal Muhammad Amin& Husayn, Rida Hasan. Morphology and molecular studies of Contracaecum larvae (nematoda : anisakidae) in some fish species from Sulaimani province, Kurdistan region, Iraq. Basrah Journal of Agrricultural Science. 2021. Vol. 34, no. 1, pp.93-110.
https://search.emarefa.net/detail/BIM-1258835
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 107-109
Record ID
BIM-1258835