The determination of the genetic distance of various snake melon Cucumis melo var. Flexuosus cultivars using inter simple sequence repeat technique (Issr)‎

Abstract EN

The experiment have been done on the winter farming season 2020 in one of the farms that belongs to Faris company in Basrah governorate, the technique ISSR have been used to study the genetic distance for twenty one isolates of snake melon Cucumis melo var.

flexuosus species.

The variations between amplified samples have been revealed after running them on a gel of agarose which have been previously stained by ethidium bromide.

Five primers which gave varied product on the agarose have been selected.

Those five primers produced 713 bands, both primers UBC 813 and UBC 815 showed the higher numbers of bands reached to 177 while the primer UBC862 showed the least numbers of bands (100) and the bands which showed multi-variations showed (46) bands, and the results primers amplification unique bands their number reached to (14) and five of those bands belong to the primer UBC842 while the primer UBC862 produced three bands while the primer UBC807 did not produced any bands.

While it has shown 100 % polymorphisms with the primers 813, 815, 842 and 862, and the least polymorphism percentage have shown with the primer UBC807 reached 75 %.

According to the efficiency primers, the highest efficiency percentage shown with the primer UBC813 and 815 reached to 24.82 % and the least percentage shown 14.02 % by the primer UBC862.

Cluster analysis showed the effect on the variance of the studied cultivars.