Purification and some properties of fungal xylanase
Other Title(s)
تنقية أنزيم الزيالنيز الفطري و بعض خصائصه
Joint Authors
Abd al-Aziz, Marwah Hamdi Mahmud
Abu al-Qasim, Nabil
Mansur, Said Muhammad
Abd al-Alim, Yasir Fathi
Source
Fayoum Journal of Agricultural Research and Development
Issue
Vol. 35, Issue 1 (31 Jan. 2021), pp.41-56, 16 p.
Publisher
Fayoum University Faculty of Agriculture
Publication Date
2021-01-31
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Topics
Abstract EN
Partial purification of xylanase produced by Aspergillus Niger strain AUMC 14230 is carried out with 80 % saturation of ammonium sulfate under pH5.0 as first step.
The second step in partial purification is Acetone 2.5 / 1 (v / v).
The last step is gill filtrations which give the highest specific from both enzymes.
Many factors affecting on purified enzymes.
The best activity of xylanase was produced till 72 hrs and decreased by (8.8 % and 10.9 %), respectively after 72 hrs.
Xylan (1mg) concentration was the best substrate to produce relative activity 100% for xylanase enzyme.
We found that the glucose has an inhibitory effect on both enzymes activity.
Optimum temperature for xylanase is 50oC, increased temperature decreased productivity of both enzymes.
The addition of Ca2+, Ba2+, Mg2+ and EDTA strongly enhanced both xylanase activity, While, Co2+ and Ag+ decreased the productivity.
On the other hand, the Fe2+, Cu2+ and Mn2+ showed moderate inhibition.
American Psychological Association (APA)
Abd al-Aziz, Marwah Hamdi Mahmud& Abu al-Qasim, Nabil& Mansur, Said Muhammad& Abd al-Alim, Yasir Fathi. 2021. Purification and some properties of fungal xylanase. Fayoum Journal of Agricultural Research and Development،Vol. 35, no. 1, pp.41-56.
https://search.emarefa.net/detail/BIM-1262391
Modern Language Association (MLA)
Abu al-Qasim, Nabil…[et al.]. Purification and some properties of fungal xylanase. Fayoum Journal of Agricultural Research and Development Vol. 35, no. 1 (2021), pp.41-56.
https://search.emarefa.net/detail/BIM-1262391
American Medical Association (AMA)
Abd al-Aziz, Marwah Hamdi Mahmud& Abu al-Qasim, Nabil& Mansur, Said Muhammad& Abd al-Alim, Yasir Fathi. Purification and some properties of fungal xylanase. Fayoum Journal of Agricultural Research and Development. 2021. Vol. 35, no. 1, pp.41-56.
https://search.emarefa.net/detail/BIM-1262391
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 53-55
Record ID
BIM-1262391