Molecular identification of local isolated streptomyces species from north region soil in iraq
Other Title(s)
التشخيص الجزيئي لأنواع Streptomyces المعزولة محليا من ترب المنطقة الشمالية في العراق
Joint Authors
Ghazi, Umar Faysal
al-Ubaydi, Safa Ismail
Source
التربية و العلم : مجلة علمية للبحوث العلمية الأساسية
Publisher
University of Mosul College of Education for Pure Science
Publication Date
2021-09-30
Country of Publication
Iraq
No. of Pages
18
Main Subjects
Biology
Earth Sciences, Water and Environment
Topics
Arabic Abstract
تم في هذه الدراسة، الحصول على 50 عزلة بكتريا من جنس Streptomyces أخذت من 30 عينة تربة، جمعت من مواقع مختلفة من شمال العراق.
عزلت العينات باستخدام اكار كازائين - النشأ و شخصت مايكروسكوبيا و مورفولوجيا بواسطة صبغة كرام والشريحة الزجاجية.
تحليل التتابع 16 srRNA استخدم للكشف عن 11 عزلة من جنس Streptomyces تم اختيار 10 حزم من الـ DNA كنتيجة للتفاعل البوليمري المتسلسل المتخصص، انتخبت العزلات المحلية المعزولة عند 1000 قاعدة نيتروجينية، تم كشف عن البكتريا قيد الاختبار باستخدام برنامج DNA Blast NCBI و كانت النتائج على النحو التالي:Streptomyces gancidicus, S.
werraensis, S.
griseorubens, S.
hawaiiensis, S.
thermocarboxydus, S.
cyaneus, S.
misionensis, S.
bellus, S.
parvulus, S.
labedae.
English Abstract
In this study Streptomyces were isolated from 50 bacterial isolates taken from 30 soil samples, these samples were collected from various locations in Iraq's various regions.
The species of Streptomyces were isolated using starch casein agar and diagnosed microscopically and morphologically by Gram staining and glass slide.
The sequence analysis 16S rRNA is used to report 11 Streptomyces.
10 bands of DNA gene, a result of specific polymerase chain reaction PCR, are elected from bacterial local isolates where 1000 base pairs within one volume, The PCR products of DNA samples were chosen from 11 local isolates based on nitrogenous base sequences.
These organisms are revealed as a result of the study and by using DNA Blast NCBI as fellows; Streptomyces gancidicus, S.
werraensis, S.
griseorubens, S.
hawaiiensis, S.
thermocarboxydus, S.
cyaneus, S.
misionensis, S.
bellus, S.
parvulus, S.
In this study Streptomyces were isolated from 50 bacterial isolates taken from 30 soil samples, these samples were collected from various locations in Iraq's various regions.
The species of Streptomyces were isolated using starch casein agar and diagnosed microscopically and morphologically by Gram staining and glass slide.
The sequence analysis 16S rRNA is used to report 11 Streptomyces.
10 bands of DNA gene, a result of specific polymerase chain reaction PCR, are elected from bacterial local isolates where 1000 base pairs within one volume, The PCR products of DNA samples were chosen from 11 local isolates based on nitrogenous base sequences.
These organisms are revealed as a result of the study and by using DNA Blast NCBI as fellows; Streptomyces gancidicus, S.
werraensis, S.
griseorubens, S.
hawaiiensis, S.
thermocarboxydus, S.
cyaneus, S.
misionensis, S.
bellus, S.
parvulus, S.
labedae,
Data Type
Conference Papers
Record ID
BIM-1266037
American Psychological Association (APA)
Ghazi, Umar Faysal& al-Ubaydi, Safa Ismail. 2021-09-30. Molecular identification of local isolated streptomyces species from north region soil in iraq. بحوث علوم الحياة عطاء مستمر رغم جائحة كورونا (3 : 2021 : جامعة الموصل، العراق). . Vol. 30, no. 3 (Special issue conference) (2021), pp.45-62.الموصل، العراق : جامعة الموصل، كلية التربية للعلوم الصرفة،.
https://search.emarefa.net/detail/BIM-1266037
Modern Language Association (MLA)
Ghazi, Umar Faysal& al-Ubaydi, Safa Ismail. Molecular identification of local isolated streptomyces species from north region soil in iraq. . الموصل، العراق : جامعة الموصل، كلية التربية للعلوم الصرفة،. 2021-09-30.
https://search.emarefa.net/detail/BIM-1266037
American Medical Association (AMA)
Ghazi, Umar Faysal& al-Ubaydi, Safa Ismail. Molecular identification of local isolated streptomyces species from north region soil in iraq. . بحوث علوم الحياة عطاء مستمر رغم جائحة كورونا (3 : 2021 : جامعة الموصل، العراق).
https://search.emarefa.net/detail/BIM-1266037
